Clinical evaluation of Bio-Rad MRSASelect™ medium for the detection of livestock-associated methicillin-resistant Staphylococcus aureus

E. Verkade; C. Verhulst; B. Van Cleef; J. Kluytmans

doi:10.1007/s10096-010-1035-7

Clinical evaluation of Bio-Rad MRSASelect™ medium for the detection of livestock-associated methicillin-resistant Staphylococcus aureus

E. Verkade^*, C. Verhulst, B. Van Cleef, J. Kluytmans

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Bio-Rad MRSASelect™ medium was evaluated for its ability to recover methicillin-resistant Staphylococcus aureus (MRSA) from nasal samples of pig farmers and their household members. In total, 257 samples were inoculated on Bio-Rad MRSASelect™ medium with and without broth enrichment and on bioMérieux MRSA ID with broth enrichment. A sample was considered to be positive if at least one of the media grew MRSA. The sensitivity of Bio-Rad MRSASelect™ medium without broth enrichment was 63.9%. With broth enrichment, the sensitivity increased to 98.4%. The specificity was 95.4% both with and without broth enrichment. In conclusion, Bio-Rad MRSASelect™ medium as well as MRSA ID medium are reliable methods to detect MRSA carriage when used in combination with broth enrichment. The directly inoculated MRSASelect™ medium was statistically significantly less sensitive than the two media after broth enrichment.

Original language	English
Pages (from-to)	109-112
Number of pages	4
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	30
Issue number	1
DOIs	https://doi.org/10.1007/s10096-010-1035-7
Publication status	Published - Jan 2011
Externally published	Yes

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title = "Clinical evaluation of Bio-Rad MRSASelect{\texttrademark} medium for the detection of livestock-associated methicillin-resistant Staphylococcus aureus",

abstract = "Bio-Rad MRSASelect{\texttrademark} medium was evaluated for its ability to recover methicillin-resistant Staphylococcus aureus (MRSA) from nasal samples of pig farmers and their household members. In total, 257 samples were inoculated on Bio-Rad MRSASelect{\texttrademark} medium with and without broth enrichment and on bioM{\'e}rieux MRSA ID with broth enrichment. A sample was considered to be positive if at least one of the media grew MRSA. The sensitivity of Bio-Rad MRSASelect{\texttrademark} medium without broth enrichment was 63.9%. With broth enrichment, the sensitivity increased to 98.4%. The specificity was 95.4% both with and without broth enrichment. In conclusion, Bio-Rad MRSASelect{\texttrademark} medium as well as MRSA ID medium are reliable methods to detect MRSA carriage when used in combination with broth enrichment. The directly inoculated MRSASelect{\texttrademark} medium was statistically significantly less sensitive than the two media after broth enrichment.",

author = "E. Verkade and C. Verhulst and {Van Cleef}, B. and J. Kluytmans",

year = "2011",

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doi = "10.1007/s10096-010-1035-7",

language = "English",

volume = "30",

pages = "109--112",

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}

Clinical evaluation of Bio-Rad MRSASelect™ medium for the detection of livestock-associated methicillin-resistant Staphylococcus aureus. / Verkade, E.; Verhulst, C.; Van Cleef, B. et al.
In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 30, No. 1, 01.2011, p. 109-112.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Clinical evaluation of Bio-Rad MRSASelect™ medium for the detection of livestock-associated methicillin-resistant Staphylococcus aureus

AU - Verkade, E.

AU - Verhulst, C.

AU - Van Cleef, B.

AU - Kluytmans, J.

PY - 2011/1

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N2 - Bio-Rad MRSASelect™ medium was evaluated for its ability to recover methicillin-resistant Staphylococcus aureus (MRSA) from nasal samples of pig farmers and their household members. In total, 257 samples were inoculated on Bio-Rad MRSASelect™ medium with and without broth enrichment and on bioMérieux MRSA ID with broth enrichment. A sample was considered to be positive if at least one of the media grew MRSA. The sensitivity of Bio-Rad MRSASelect™ medium without broth enrichment was 63.9%. With broth enrichment, the sensitivity increased to 98.4%. The specificity was 95.4% both with and without broth enrichment. In conclusion, Bio-Rad MRSASelect™ medium as well as MRSA ID medium are reliable methods to detect MRSA carriage when used in combination with broth enrichment. The directly inoculated MRSASelect™ medium was statistically significantly less sensitive than the two media after broth enrichment.

AB - Bio-Rad MRSASelect™ medium was evaluated for its ability to recover methicillin-resistant Staphylococcus aureus (MRSA) from nasal samples of pig farmers and their household members. In total, 257 samples were inoculated on Bio-Rad MRSASelect™ medium with and without broth enrichment and on bioMérieux MRSA ID with broth enrichment. A sample was considered to be positive if at least one of the media grew MRSA. The sensitivity of Bio-Rad MRSASelect™ medium without broth enrichment was 63.9%. With broth enrichment, the sensitivity increased to 98.4%. The specificity was 95.4% both with and without broth enrichment. In conclusion, Bio-Rad MRSASelect™ medium as well as MRSA ID medium are reliable methods to detect MRSA carriage when used in combination with broth enrichment. The directly inoculated MRSASelect™ medium was statistically significantly less sensitive than the two media after broth enrichment.

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JO - European Journal of Clinical Microbiology and Infectious Diseases

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Clinical evaluation of Bio-Rad MRSASelect™ medium for the detection of livestock-associated methicillin-resistant Staphylococcus aureus

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