TY - JOUR
T1 - Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury
AU - Deddens, Janine C
AU - Vrijsen, Krijn R
AU - Colijn, Johanna M
AU - Oerlemans, Marish
AU - Metz, Corina H G
AU - van der Vlist, Els J
AU - Nolte-'t Hoen, Esther N M
AU - den Ouden, Krista
AU - Jansen of Lorkeers, SJ
AU - van der Spoel, TIG
AU - Koudstaal, Stefan
AU - Arkesteijn, Ger J
AU - Wauben, Marca H M
AU - van Laake, Linda W
AU - Doevendans, Pieter A
AU - Chamuleau, Steven A J
AU - Sluijter, Joost P G
PY - 2016/7/6
Y1 - 2016/7/6
N2 - Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon MI. Therefore, we studied the temporal release, potential source, and transportation of circulating miRNAs in different models of ischemia reperfusion (I/R) injury. We demonstrated that extracellular vesicles are released from the ischemic myocardium upon I/R injury. Moreover, we provided evidence that cardiac and muscle-specific miRNAs are transported by extracellular vesicles and are rapidly detectable in plasma. Since these vesicles are enriched for the released miRNAs and their detection precedes traditional damage markers, they hold great potential as specific early biomarkers for MI.
AB - Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon MI. Therefore, we studied the temporal release, potential source, and transportation of circulating miRNAs in different models of ischemia reperfusion (I/R) injury. We demonstrated that extracellular vesicles are released from the ischemic myocardium upon I/R injury. Moreover, we provided evidence that cardiac and muscle-specific miRNAs are transported by extracellular vesicles and are rapidly detectable in plasma. Since these vesicles are enriched for the released miRNAs and their detection precedes traditional damage markers, they hold great potential as specific early biomarkers for MI.
KW - Biomarkers
KW - Circulating microRNA
KW - Exosomes
KW - Extracellular vesicles
KW - Myocardial infarction
UR - http://www.scopus.com/inward/record.url?scp=84978139021&partnerID=8YFLogxK
U2 - 10.1007/s12265-016-9705-1
DO - 10.1007/s12265-016-9705-1
M3 - Article
C2 - 27383837
SN - 1937-5387
VL - 9
SP - 291
EP - 301
JO - Journal of Cardiovascular Translational Research
JF - Journal of Cardiovascular Translational Research
IS - 4
ER -