Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

Jiayi Pei; Noortje A M van den Dungen; Folkert W Asselbergs; Michal Mokry; Magdalena Harakalova

doi:10.1007/978-1-0716-2140-0_6

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

Jiayi Pei, Noortje A M van den Dungen, Folkert W Asselbergs, Michal Mokry, Magdalena Harakalova

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.

Original language	English
Title of host publication	Chromatin
Subtitle of host publication	Methods and Protocols
Editors	Julia Horsfield, Judith Marsman
Publisher	Humana Press
Chapter	6
Pages	97-111
Number of pages	15
Volume	2458
ISBN (Electronic)	978-1-0716-2140-0
ISBN (Print)	978-1-0716-2139-4
DOIs	https://doi.org/10.1007/978-1-0716-2140-0_6
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2458
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Antibody
Cardiac tissues
Chromatin immunoprecipitation
Enhancers
Promoters
Sequencing
Small biopsy

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10.1007/978-1-0716-2140-0_6

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Pei, J., van den Dungen, N. A. M., Asselbergs, F. W., Mokry, M., & Harakalova, M. (2022). Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue. In J. Horsfield, & J. Marsman (Eds.), Chromatin: Methods and Protocols (Vol. 2458, pp. 97-111). (Methods in Molecular Biology; Vol. 2458). Humana Press. https://doi.org/10.1007/978-1-0716-2140-0_6

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title = "Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue",

abstract = "Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.",

keywords = "Antibody, Cardiac tissues, Chromatin immunoprecipitation, Enhancers, Promoters, Sequencing, Small biopsy",

author = "Jiayi Pei and \{van den Dungen\}, \{Noortje A M\} and Asselbergs, \{Folkert W\} and Michal Mokry and Magdalena Harakalova",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2140-0\_6",

language = "English",

isbn = "978-1-0716-2139-4",

volume = "2458",

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Pei, J, van den Dungen, NAM , Asselbergs, FW , Mokry, M & Harakalova, M 2022, Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue. in J Horsfield & J Marsman (eds), Chromatin: Methods and Protocols. vol. 2458, Methods in Molecular Biology, vol. 2458, Humana Press, pp. 97-111. https://doi.org/10.1007/978-1-0716-2140-0_6

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue. / Pei, Jiayi; van den Dungen, Noortje A M ; Asselbergs, Folkert W et al.
Chromatin: Methods and Protocols. ed. / Julia Horsfield; Judith Marsman. Vol. 2458 Humana Press, 2022. p. 97-111 (Methods in Molecular Biology; Vol. 2458).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

TY - CHAP

T1 - Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

AU - Pei, Jiayi

AU - van den Dungen, Noortje A M

AU - Asselbergs, Folkert W

AU - Mokry, Michal

AU - Harakalova, Magdalena

PY - 2022

Y1 - 2022

N2 - Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.

AB - Chromatin immunoprecipitation and sequencing (ChIP-seq) is a well-established method to study the epigenetic profile at the genome-wide scale, including histone modifications and DNA-protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.

KW - Antibody

KW - Cardiac tissues

KW - Chromatin immunoprecipitation

KW - Enhancers

KW - Promoters

KW - Sequencing

KW - Small biopsy

UR - https://www.scopus.com/pages/publications/85123963418

U2 - 10.1007/978-1-0716-2140-0_6

DO - 10.1007/978-1-0716-2140-0_6

M3 - Chapter

C2 - 35103964

SN - 978-1-0716-2139-4

VL - 2458

T3 - Methods in Molecular Biology

SP - 97

EP - 111

BT - Chromatin

A2 - Horsfield, Julia

A2 - Marsman, Judith

PB - Humana Press

ER -

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue

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