Characterization of pKP-M1144, a Novel ColE1-like plasmid encoding IMP-8, GES-5, and BEL-1 β-lactamases, from a Klebsiella pneumoniae sequence type 252 isolate

Costas C. Papagiannitsis*, Monika Dolejska, Radoslaw Izdebski, Hana Dobiasova, Vendula Studentova, Francisco J. Esteves, Lennie P G Derde, Marc J M Bonten, Jaroslav Hrabák, Marek Gniadkowski

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

IMP-8 metallo-β-lactamase was identified in Klebsiella pneumoniae sequence type 252 (ST252), isolated in a Portuguese hospital in 2009. bla<inf>IMP-8</inf> was the first gene cassette of a novel class 3 integron, In1144, also carrying the bla<inf>GES-5</inf>, bla<inf>BEL-1</inf>, and aacA4 cassettes. In1144 was located on a ColE1-like plasmid, pKP-M1144 (12,029 bp), with a replication region of limited nucleotide similarity to those of other RNA-priming plasmids, such as pJHCMW1. In1144 and pKP-M1144 represent an interesting case of evolution of resistance determinants in Gram-negative bacteria.

Original languageEnglish
Pages (from-to)5065-5068
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number8
DOIs
Publication statusPublished - 1 Aug 2015

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