TY - JOUR
T1 - Characterization of antisera raised against hypocalcin (teleocalcin) purified from corpuscles of stannius of rainbow trout, Salmo gairdneri
AU - Kaneko, Toyoji
AU - Fraser, Robert A.
AU - Labedz, Teresa
AU - Harvey, Stephen
AU - Lafeber, Floris P.J.G.
AU - Pang, Peter K.T.
N1 - Funding Information:
We thank the National Sciences and Engineering Research Council of Canada (Grant NSERC A36391 and the Alberta Heritage Foundation for Medical Research (Grant AHFMR EG 6972) for their generous support of our work. Teleocalcin used in the present study was kindly provided by Dr. D. H. Copp, Department of Physiology, University of British Columbia, Vancouver. We also thank Dr. S. E. Wendelaar Bonga for providing hypocalcin.
PY - 1988/2
Y1 - 1988/2
N2 - Highly specific antisera were raised against hypocalcin, a 54-kDa glycoprotein purified from the corpuscles of Stannius (CS) of rainbow trout, Salmo gairdneri. The specificity of the antisera was determined by using Ouchterlony's double immunodiffusion test, radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and immunocytochemistry. In the double immunodiffusion test, a single precipitin line was formed between the antisera and hypocalcin. Both RIA and ELISA studies showed that serial dilutions of hypocalcin, teleocalcin, and CS extracts produced dose-response curves, whereas rat FSH, chicken LH, bovine TSH, salmon calcitonin, bovine parathyroid hormone, and human angiotensins I and II failed to cross-react with the antiserum. Immunoreactive hypocalcin was demonstrated in the plasma of a teleost (flounder), but not of an elasmobranch (dogfish). In the immunocytochemistry, most of the gland cells showed strong immunoreaction with the antisera, whereas some cells displayed no immunoreactivity.
AB - Highly specific antisera were raised against hypocalcin, a 54-kDa glycoprotein purified from the corpuscles of Stannius (CS) of rainbow trout, Salmo gairdneri. The specificity of the antisera was determined by using Ouchterlony's double immunodiffusion test, radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and immunocytochemistry. In the double immunodiffusion test, a single precipitin line was formed between the antisera and hypocalcin. Both RIA and ELISA studies showed that serial dilutions of hypocalcin, teleocalcin, and CS extracts produced dose-response curves, whereas rat FSH, chicken LH, bovine TSH, salmon calcitonin, bovine parathyroid hormone, and human angiotensins I and II failed to cross-react with the antiserum. Immunoreactive hypocalcin was demonstrated in the plasma of a teleost (flounder), but not of an elasmobranch (dogfish). In the immunocytochemistry, most of the gland cells showed strong immunoreaction with the antisera, whereas some cells displayed no immunoreactivity.
UR - http://www.scopus.com/inward/record.url?scp=0023838272&partnerID=8YFLogxK
U2 - 10.1016/0016-6480(88)90011-1
DO - 10.1016/0016-6480(88)90011-1
M3 - Article
C2 - 3130286
AN - SCOPUS:0023838272
SN - 0016-6480
VL - 69
SP - 238
EP - 245
JO - General and comparative endocrinology
JF - General and comparative endocrinology
IS - 2
ER -