Cell‐mediated cytotoxicity toward class I and class antigens on canine T and B lymphoblasts

W. A. Buurman; C. J van der Linden; A. J J M Daemen; G. Groenewegen; R. F C Doveren; G. Kootstra

doi:10.1111/j.1399-0039.1984.tb00397.x

Cell‐mediated cytotoxicity toward class I and class antigens on canine T and B lymphoblasts

W. A. Buurman^*, C. J van der Linden, A. J J M Daemen, G. Groenewegen, R. F C Doveren, G. Kootstra

^*Corresponding author for this work

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Abstract

Cellular cytotoxicity generated in vitro has been studied with canine T and B lymphoblasts as target cells. B lymphocytes were isolated by resetting with protein A labeled sheep red blood cells and stimulated with sepharose bound protein A. Such highly purified stimulated B lymphoblasts appeared to be useful target cells for cellular cytotoxicity. Cold target inhibition studies and limiting dilution analysis revealed that B lymphocytes did not carry targets for cytotoxic T lymphocytes (CTL) which were not present on PHA stimulated T lymphoblasts.

Original language	English
Pages (from-to)	48-57
Number of pages	10
Journal	Tissue Antigens
Volume	24
Issue number	1
DOIs	https://doi.org/10.1111/j.1399-0039.1984.tb00397.x
Publication status	Published - 1 Jan 1984

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abstract = "Cellular cytotoxicity generated in vitro has been studied with canine T and B lymphoblasts as target cells. B lymphocytes were isolated by resetting with protein A labeled sheep red blood cells and stimulated with sepharose bound protein A. Such highly purified stimulated B lymphoblasts appeared to be useful target cells for cellular cytotoxicity. Cold target inhibition studies and limiting dilution analysis revealed that B lymphocytes did not carry targets for cytotoxic T lymphocytes (CTL) which were not present on PHA stimulated T lymphoblasts.",

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AU - Buurman, W. A.

AU - Linden, C. J van der

AU - Daemen, A. J J M

AU - Groenewegen, G.

AU - Doveren, R. F C

AU - Kootstra, G.

PY - 1984/1/1

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AB - Cellular cytotoxicity generated in vitro has been studied with canine T and B lymphoblasts as target cells. B lymphocytes were isolated by resetting with protein A labeled sheep red blood cells and stimulated with sepharose bound protein A. Such highly purified stimulated B lymphoblasts appeared to be useful target cells for cellular cytotoxicity. Cold target inhibition studies and limiting dilution analysis revealed that B lymphocytes did not carry targets for cytotoxic T lymphocytes (CTL) which were not present on PHA stimulated T lymphoblasts.

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ER -

Cell‐mediated cytotoxicity toward class I and class antigens on canine T and B lymphoblasts

Abstract

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