C/EBP regulates the promoter of the eosinophil-derived neurotoxin/RNS2 gene in human eosinophilic cells

Belinda Baltus; Miranda Buitenhuis; Thamar B. Van Dijk; Charles Vinson; Jan A.M. Raaijmakers; Jan Willem J. Lammers; Leo Koenderman; Rolf P. De Groot

doi:10.1002/jlb.66.4.683

C/EBP regulates the promoter of the eosinophil-derived neurotoxin/RNS2 gene in human eosinophilic cells

Belinda Baltus, Miranda Buitenhuis, Thamar B. Van Dijk, Charles Vinson, Jan A.M. Raaijmakers, Jan Willem J. Lammers, Leo Koenderman, Rolf P. De Groot^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

The eosinophil-derived neurotoxin (EDN), a member of the mammalian ribonuclease family, is found in the large specific granules of human eosinophilic leukocytes. We have investigated the role of the C/EBP transcription factor family in the regulation of EDN promoter activity. Here we show that the C/EBP family is involved in intrinsic regulation of EDN promoter activity. We have identified a C/EBP binding site located at -124 in the proximal promoter of the EDN gene. Mutation of this C/EBP site results in a decrease of promoter activity in HL-60-eos cells as well as in eosinophils differentiated in vitro from CD34⁺ cells. Different C/EBP proteins are able to bind to the C/EBP site as shown by gel shift assay. Our results indicate the importance of the C/EBP family in the regulation of the EDN gene in eosinophils.

Original language	English
Pages (from-to)	683-688
Number of pages	6
Journal	Journal of Leukocyte Biology
Volume	66
Issue number	4
DOIs	https://doi.org/10.1002/jlb.66.4.683
Publication status	Published - 1 Jan 1999

Keywords

Granulocytes
Transcription regulation

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title = "C/EBP regulates the promoter of the eosinophil-derived neurotoxin/RNS2 gene in human eosinophilic cells",

abstract = "The eosinophil-derived neurotoxin (EDN), a member of the mammalian ribonuclease family, is found in the large specific granules of human eosinophilic leukocytes. We have investigated the role of the C/EBP transcription factor family in the regulation of EDN promoter activity. Here we show that the C/EBP family is involved in intrinsic regulation of EDN promoter activity. We have identified a C/EBP binding site located at -124 in the proximal promoter of the EDN gene. Mutation of this C/EBP site results in a decrease of promoter activity in HL-60-eos cells as well as in eosinophils differentiated in vitro from CD34+ cells. Different C/EBP proteins are able to bind to the C/EBP site as shown by gel shift assay. Our results indicate the importance of the C/EBP family in the regulation of the EDN gene in eosinophils.",

keywords = "Granulocytes, Transcription regulation",

author = "Belinda Baltus and Miranda Buitenhuis and {Van Dijk}, {Thamar B.} and Charles Vinson and Raaijmakers, {Jan A.M.} and Lammers, {Jan Willem J.} and Leo Koenderman and {De Groot}, {Rolf P.}",

year = "1999",

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doi = "10.1002/jlb.66.4.683",

language = "English",

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T1 - C/EBP regulates the promoter of the eosinophil-derived neurotoxin/RNS2 gene in human eosinophilic cells

AU - Baltus, Belinda

AU - Buitenhuis, Miranda

AU - Van Dijk, Thamar B.

AU - Vinson, Charles

AU - Raaijmakers, Jan A.M.

AU - Lammers, Jan Willem J.

AU - Koenderman, Leo

AU - De Groot, Rolf P.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The eosinophil-derived neurotoxin (EDN), a member of the mammalian ribonuclease family, is found in the large specific granules of human eosinophilic leukocytes. We have investigated the role of the C/EBP transcription factor family in the regulation of EDN promoter activity. Here we show that the C/EBP family is involved in intrinsic regulation of EDN promoter activity. We have identified a C/EBP binding site located at -124 in the proximal promoter of the EDN gene. Mutation of this C/EBP site results in a decrease of promoter activity in HL-60-eos cells as well as in eosinophils differentiated in vitro from CD34+ cells. Different C/EBP proteins are able to bind to the C/EBP site as shown by gel shift assay. Our results indicate the importance of the C/EBP family in the regulation of the EDN gene in eosinophils.

AB - The eosinophil-derived neurotoxin (EDN), a member of the mammalian ribonuclease family, is found in the large specific granules of human eosinophilic leukocytes. We have investigated the role of the C/EBP transcription factor family in the regulation of EDN promoter activity. Here we show that the C/EBP family is involved in intrinsic regulation of EDN promoter activity. We have identified a C/EBP binding site located at -124 in the proximal promoter of the EDN gene. Mutation of this C/EBP site results in a decrease of promoter activity in HL-60-eos cells as well as in eosinophils differentiated in vitro from CD34+ cells. Different C/EBP proteins are able to bind to the C/EBP site as shown by gel shift assay. Our results indicate the importance of the C/EBP family in the regulation of the EDN gene in eosinophils.

KW - Granulocytes

KW - Transcription regulation

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U2 - 10.1002/jlb.66.4.683

DO - 10.1002/jlb.66.4.683

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AN - SCOPUS:0032865919

SN - 0741-5400

VL - 66

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EP - 688

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

IS - 4

ER -

C/EBP regulates the promoter of the eosinophil-derived neurotoxin/RNS2 gene in human eosinophilic cells

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Keywords

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