TY - JOUR
T1 - cDC2 and plasmacytoid dendritic cells diminish from tissues of patients with non-Hodgkin orbital lymphoma and idiopathic orbital inflammation
AU - Laban, Kamil G.
AU - Rijken, Rianne
AU - Hiddingh, Sanne
AU - Mertens, Jorre S.
AU - van der Veen, Rob L.P.
AU - Eenhorst, Christine A.E.
AU - Pandit, Aridaman
AU - Radstake, Timothy R.D.J.
AU - de Boer, Joke H.
AU - Kalmann, Rachel
AU - Kuiper, Jonas J.W.
N1 - Funding Information:
We would like to thank Maarten Mourits and Stijn Genders for their assistance in the recruitment of patients. This study was supported by unrestricted grants from the Dr. F.P Fischerstichting, Stichting Lijf & Leven, Rotterdamse Stichting Blindenbelangen, and Stichting Ankie Hak. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funding Information:
We would like to thank Maarten Mourits and Stijn Genders for their assistance in the recruitment of patients. This study was supported by unrestricted grants from the Dr. F.P Fischerstichting, Stichting Lijf & Leven, Rotterdamse Stichting Blindenbelangen, and Stichting Ankie Hak. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2019 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2020/4
Y1 - 2020/4
N2 - Non-Hodgkin orbital lymphoma (NHOL) and idiopathic orbital inflammation (IOI) are common orbital conditions with largely unknown pathophysiology. To investigate the immune cell composition of these diseases, we performed standardized 29 parameter flow cytometry phenotyping in peripheral blood mononuclear cells of 18 NHOL patients, 21 IOI patients, and 41 unaffected controls. Automatic gating by FlowSOM revealed decreased abundance of meta-clusters containing dendritic cells in patients, which we confirmed by manual gating. A decreased percentage of (HLA-DR+CD303+CD123+) plasmacytoid dendritic cells (pDC) in the circulation of IOI patients and decreased (HLA-DR+CD11c+CD1c+) conventional dendritic cells (cDC) type-2 for IOI patients were replicated in an independent cohort of patients and controls. Meta-analysis of both cohorts demonstrated that pDCs are also decreased in blood of NHOL patients and highlighted that the decrease in blood cDC type-2 was specific for IOI patients compared to NHOL or controls. Deconvolution-based estimation of immune cells in transcriptomic data of 48 orbital biopsies revealed a decrease in the abundance of pDC and cDC populations within the orbital microenvironment of IOI patients. Collectively, these data suggest a previously underappreciated role for dendritic cells in orbital disorders.
AB - Non-Hodgkin orbital lymphoma (NHOL) and idiopathic orbital inflammation (IOI) are common orbital conditions with largely unknown pathophysiology. To investigate the immune cell composition of these diseases, we performed standardized 29 parameter flow cytometry phenotyping in peripheral blood mononuclear cells of 18 NHOL patients, 21 IOI patients, and 41 unaffected controls. Automatic gating by FlowSOM revealed decreased abundance of meta-clusters containing dendritic cells in patients, which we confirmed by manual gating. A decreased percentage of (HLA-DR+CD303+CD123+) plasmacytoid dendritic cells (pDC) in the circulation of IOI patients and decreased (HLA-DR+CD11c+CD1c+) conventional dendritic cells (cDC) type-2 for IOI patients were replicated in an independent cohort of patients and controls. Meta-analysis of both cohorts demonstrated that pDCs are also decreased in blood of NHOL patients and highlighted that the decrease in blood cDC type-2 was specific for IOI patients compared to NHOL or controls. Deconvolution-based estimation of immune cells in transcriptomic data of 48 orbital biopsies revealed a decrease in the abundance of pDC and cDC populations within the orbital microenvironment of IOI patients. Collectively, these data suggest a previously underappreciated role for dendritic cells in orbital disorders.
KW - cDC2
KW - FlowSOM
KW - idiopathic orbital inflammation
KW - non-Hodgkin orbital lymphoma
KW - plasmacytoid dendritic cells
UR - https://www.scopus.com/pages/publications/85078064220
U2 - 10.1002/eji.201948370
DO - 10.1002/eji.201948370
M3 - Article
C2 - 31841217
AN - SCOPUS:85078064220
SN - 0014-2980
VL - 50
SP - 548
EP - 557
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 4
ER -