CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes

R J Rentenaar; P C Wever; F N van Diepen; P T Schellekens; P M Wertheim; I J ten Berge

doi:10.1093/ndt/14.6.1430

CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes

R J Rentenaar, P C Wever, F N van Diepen, P T Schellekens, P M Wertheim, I J ten Berge

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: T-lymphocytes that co-express CD4 and CD8 antigens may be found in small percentages in the peripheral blood of healthy individuals, and have a CD4brightCD8dull phenotype. CD4dullCD8bright T-lymphocytes have been found only in temporal association with some viral infections.

METHODS: Four-colour flow cytometric analysis of peripheral blood mononuclear cells from a renal transplant recipient with cytomegalovirus infection was performed.

RESULTS: A small but clearly distinguishable subpopulation of CD4dullCD8bright double-positive T-lymphocytes was detected, that exhibited phenotypic characteristics of cytotoxic T-lymphocytes and were granzyme B positive. Furthermore, no naive cells appeared to be present within this subpopulation.

CONCLUSIONS: CD4dullCD8bright double-positive T-lymphocytes are enriched for memory and effector cytotoxic T cells.

Original language	English
Pages (from-to)	1430-1434
Number of pages	5
Journal	Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
Volume	14
Issue number	6
DOIs	https://doi.org/10.1093/ndt/14.6.1430
Publication status	Published - Jun 1999
Externally published	Yes

Keywords

CD4 Antigens/analysis
CD8 Antigens/analysis
Female
Granzymes
Humans
Immunologic Memory
Immunophenotyping
Middle Aged
Serine Endopeptidases/metabolism
T-Lymphocytes/immunology

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10.1093/ndt/14.6.1430

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Rentenaar, R. J., Wever, P. C., van Diepen, F. N., Schellekens, P. T., Wertheim, P. M., & ten Berge, I. J. (1999). CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 14(6), 1430-1434. https://doi.org/10.1093/ndt/14.6.1430

@article{ca93eb68666248debaece7bd4a6d7e90,

title = "CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes",

abstract = "BACKGROUND: T-lymphocytes that co-express CD4 and CD8 antigens may be found in small percentages in the peripheral blood of healthy individuals, and have a CD4brightCD8dull phenotype. CD4dullCD8bright T-lymphocytes have been found only in temporal association with some viral infections.METHODS: Four-colour flow cytometric analysis of peripheral blood mononuclear cells from a renal transplant recipient with cytomegalovirus infection was performed.RESULTS: A small but clearly distinguishable subpopulation of CD4dullCD8bright double-positive T-lymphocytes was detected, that exhibited phenotypic characteristics of cytotoxic T-lymphocytes and were granzyme B positive. Furthermore, no naive cells appeared to be present within this subpopulation.CONCLUSIONS: CD4dullCD8bright double-positive T-lymphocytes are enriched for memory and effector cytotoxic T cells.",

keywords = "CD4 Antigens/analysis, CD8 Antigens/analysis, Female, Granzymes, Humans, Immunologic Memory, Immunophenotyping, Middle Aged, Serine Endopeptidases/metabolism, T-Lymphocytes/immunology",

author = "Rentenaar, {R J} and Wever, {P C} and {van Diepen}, {F N} and Schellekens, {P T} and Wertheim, {P M} and {ten Berge}, {I J}",

year = "1999",

month = jun,

doi = "10.1093/ndt/14.6.1430",

language = "English",

volume = "14",

pages = "1430--1434",

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issn = "0931-0509",

publisher = "Oxford University Press",

number = "6",

}

Rentenaar, RJ, Wever, PC, van Diepen, FN, Schellekens, PT, Wertheim, PM & ten Berge, IJ 1999, 'CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes', Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, vol. 14, no. 6, pp. 1430-1434. https://doi.org/10.1093/ndt/14.6.1430

CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes. / Rentenaar, R J; Wever, P C; van Diepen, F N et al.
In: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 14, No. 6, 06.1999, p. 1430-1434.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes

AU - Rentenaar, R J

AU - Wever, P C

AU - van Diepen, F N

AU - Schellekens, P T

AU - Wertheim, P M

AU - ten Berge, I J

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N2 - BACKGROUND: T-lymphocytes that co-express CD4 and CD8 antigens may be found in small percentages in the peripheral blood of healthy individuals, and have a CD4brightCD8dull phenotype. CD4dullCD8bright T-lymphocytes have been found only in temporal association with some viral infections.METHODS: Four-colour flow cytometric analysis of peripheral blood mononuclear cells from a renal transplant recipient with cytomegalovirus infection was performed.RESULTS: A small but clearly distinguishable subpopulation of CD4dullCD8bright double-positive T-lymphocytes was detected, that exhibited phenotypic characteristics of cytotoxic T-lymphocytes and were granzyme B positive. Furthermore, no naive cells appeared to be present within this subpopulation.CONCLUSIONS: CD4dullCD8bright double-positive T-lymphocytes are enriched for memory and effector cytotoxic T cells.

AB - BACKGROUND: T-lymphocytes that co-express CD4 and CD8 antigens may be found in small percentages in the peripheral blood of healthy individuals, and have a CD4brightCD8dull phenotype. CD4dullCD8bright T-lymphocytes have been found only in temporal association with some viral infections.METHODS: Four-colour flow cytometric analysis of peripheral blood mononuclear cells from a renal transplant recipient with cytomegalovirus infection was performed.RESULTS: A small but clearly distinguishable subpopulation of CD4dullCD8bright double-positive T-lymphocytes was detected, that exhibited phenotypic characteristics of cytotoxic T-lymphocytes and were granzyme B positive. Furthermore, no naive cells appeared to be present within this subpopulation.CONCLUSIONS: CD4dullCD8bright double-positive T-lymphocytes are enriched for memory and effector cytotoxic T cells.

KW - CD4 Antigens/analysis

KW - CD8 Antigens/analysis

KW - Female

KW - Granzymes

KW - Humans

KW - Immunologic Memory

KW - Immunophenotyping

KW - Middle Aged

KW - Serine Endopeptidases/metabolism

KW - T-Lymphocytes/immunology

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JO - Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

JF - Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

IS - 6

ER -

Rentenaar RJ, Wever PC, van Diepen FN, Schellekens PT, Wertheim PM, ten Berge IJ. CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 1999 Jun;14(6):1430-1434. doi: 10.1093/ndt/14.6.1430

CD4dullCD8bright double-positive T-lymphocytes have a phenotype of granzyme Bpos CD8pos memory T-lymphocytes

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