TY - JOUR
T1 - CD4+CXCR5+ T cells in chronic HCV infection produce less IL-21, yet are efficient at supporting B cell responses
AU - Spaan, Michelle
AU - Kreefft, Kim
AU - De Graav, Gretchen N.
AU - Brouwer, Willem P.
AU - De Knegt, Robert J.
AU - Ten Kate, Fibo J W
AU - Baan, Carla C.
AU - Vanwolleghem, Thomas
AU - Janssen, Harry L A
AU - Boonstra, Andre
PY - 2015
Y1 - 2015
N2 - Background & Aims: During chronic HCV infection, T cell dependent virus-specific antibodies are produced. However, the role of B-T cell interaction in chronic HCV is largely unknown. CD4+CXCR5+ T follicular helper (TFH)-cells activate B cells and are important for clearance of various chronic viral infections. We investigated the function of TFH cells and B cells in liver and in peripheral blood of chronic HCV patients. Methods: T cells from chronic HCV patients and healthy individuals were analysed for expression of CXCR5, PD-1, ICOS, and IL-21 and IFN-c production by flow cytometry. CD19+ B cell subpopulations were identified on the basis of CD27 and IgD expression. In order to assess the frequency and function of T cells and B cells in liver follicles, immunohistochemistry was performed for CD3, CXCR5, Bcl6, IL-21, CD20, IgD, IgM, and IgG. Results: The frequency of IL-21-producing CXCR5+CD4+ T cells in blood was lower in HCV patients compared to healthy individuals (p = 0.002), which was reflected by lower serum IL-21 levels (p
AB - Background & Aims: During chronic HCV infection, T cell dependent virus-specific antibodies are produced. However, the role of B-T cell interaction in chronic HCV is largely unknown. CD4+CXCR5+ T follicular helper (TFH)-cells activate B cells and are important for clearance of various chronic viral infections. We investigated the function of TFH cells and B cells in liver and in peripheral blood of chronic HCV patients. Methods: T cells from chronic HCV patients and healthy individuals were analysed for expression of CXCR5, PD-1, ICOS, and IL-21 and IFN-c production by flow cytometry. CD19+ B cell subpopulations were identified on the basis of CD27 and IgD expression. In order to assess the frequency and function of T cells and B cells in liver follicles, immunohistochemistry was performed for CD3, CXCR5, Bcl6, IL-21, CD20, IgD, IgM, and IgG. Results: The frequency of IL-21-producing CXCR5+CD4+ T cells in blood was lower in HCV patients compared to healthy individuals (p = 0.002), which was reflected by lower serum IL-21 levels (p
KW - Chronic hepatitis C
KW - IL-21
KW - Immunology
KW - Intrahepatic
KW - T follicular helper cells
UR - http://www.scopus.com/inward/record.url?scp=84922267705&partnerID=8YFLogxK
U2 - 10.1016/j.jhep.2014.09.024
DO - 10.1016/j.jhep.2014.09.024
M3 - Article
C2 - 25281860
AN - SCOPUS:84922267705
SN - 0168-8278
VL - 62
SP - 303
EP - 310
JO - Journal of Hepatology
JF - Journal of Hepatology
IS - 2
ER -