TY - JOUR
T1 - CD123 expression levels in 846 acute leukemia patients based on standardized immunophenotyping
AU - Bras, Anne E
AU - de Haas, Valerie
AU - van Stigt, Arthur
AU - Jongen-Lavrencic, Mojca
AU - Beverloo, H Berna
AU - Te Marvelde, Jeroen G
AU - Zwaan, C Michel
AU - van Dongen, Jacques J M
AU - Leusen, Jeanette H W
AU - van der Velden, Vincent H J
N1 - Funding Information:
We gratefully acknowledge all clinicians participating in this study for providing patient material and clinical data. We gratefully thank all technicians of the Laboratory Medical immunology for their support. The research for this manuscript was performed within the framework of the Erasmus Postgraduate School Molecular Medicine.
Publisher Copyright:
© 2018 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Background: While it is known that CD123 is normally strongly expressed on plasmacytoid dendritic cells and completely absent on nucleated red blood cells, detailed information regarding CD123 expression in acute leukemia is scarce and, if available, hard to compare due to different methodologies. Methods: CD123 expression was evaluated using standardized EuroFlow immunophenotyping in 139 pediatric AML, 316 adult AML, 193 pediatric BCP-ALL, 69 adult BCP-ALL, 101 pediatric T-ALL, and 28 adult T-ALL patients. Paired diagnosis-relapse samples were available for 57 AML and 19 BCP-ALL patients. Leukemic stem cell (LSC) data was available for 32 pediatric AML patients. CD123 expression was evaluated based on mean fluorescence intensity, median fluorescence intensity, and percentage CD123 positive cells. Results: EuroFlow panels were stable over time and between laboratories. CD123 was expressed in the majority of AML and BCP-ALL patients, but absent in most T-ALL patients. Within AML, CD123 expression was lower in erythroid/megakaryocytic leukemia, higher in NPM1 mutated and FLT3-ITD mutated leukemia, and comparable between LSC and leukemic blasts. Within BCP-ALL, CD123 expression was higher in patients with (high) hyperdiploid karyotypes and the BCR-ABL fusion gene. Interestingly, CD123 expression was increased in BCP-ALL relapses while highly variable in AML relapses (compared to CD123 expression at diagnosis). Conclusions: Authors evaluated CD123 expression in a large cohort of acute leukemia patients, based on standardized and reproducible methodology. Our results may facilitate stratification of patients most likely to respond to CD123 targeted therapies and serve as reference for CD123 expression (in health and disease).
AB - Background: While it is known that CD123 is normally strongly expressed on plasmacytoid dendritic cells and completely absent on nucleated red blood cells, detailed information regarding CD123 expression in acute leukemia is scarce and, if available, hard to compare due to different methodologies. Methods: CD123 expression was evaluated using standardized EuroFlow immunophenotyping in 139 pediatric AML, 316 adult AML, 193 pediatric BCP-ALL, 69 adult BCP-ALL, 101 pediatric T-ALL, and 28 adult T-ALL patients. Paired diagnosis-relapse samples were available for 57 AML and 19 BCP-ALL patients. Leukemic stem cell (LSC) data was available for 32 pediatric AML patients. CD123 expression was evaluated based on mean fluorescence intensity, median fluorescence intensity, and percentage CD123 positive cells. Results: EuroFlow panels were stable over time and between laboratories. CD123 was expressed in the majority of AML and BCP-ALL patients, but absent in most T-ALL patients. Within AML, CD123 expression was lower in erythroid/megakaryocytic leukemia, higher in NPM1 mutated and FLT3-ITD mutated leukemia, and comparable between LSC and leukemic blasts. Within BCP-ALL, CD123 expression was higher in patients with (high) hyperdiploid karyotypes and the BCR-ABL fusion gene. Interestingly, CD123 expression was increased in BCP-ALL relapses while highly variable in AML relapses (compared to CD123 expression at diagnosis). Conclusions: Authors evaluated CD123 expression in a large cohort of acute leukemia patients, based on standardized and reproducible methodology. Our results may facilitate stratification of patients most likely to respond to CD123 targeted therapies and serve as reference for CD123 expression (in health and disease).
KW - CD123
KW - acute leukemia
KW - standardized immunophenotyping
KW - targeted therapy
UR - http://www.scopus.com/inward/record.url?scp=85056771133&partnerID=8YFLogxK
U2 - 10.1002/cyto.b.21745
DO - 10.1002/cyto.b.21745
M3 - Article
C2 - 30450744
SN - 1552-4949
VL - 96
SP - 134
EP - 142
JO - Cytometry. Part B, Clinical Cytometry.
JF - Cytometry. Part B, Clinical Cytometry.
IS - 2
ER -