TY - JOUR
T1 - Cardiac optogenetics
T2 - using light to monitor cardiac physiology
AU - Koopman, Charlotte D.
AU - Zimmermann, Wolfram-Hubertus
AU - Knöpfel, Thomas
AU - de Boer, Teun P.
N1 - Publisher Copyright:
© 2017, The Author(s).
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2017/8/31
Y1 - 2017/8/31
N2 - Our current understanding of cardiac excitation and its coupling to contraction is largely based on ex vivo studies utilising fluorescent organic dyes to assess cardiac action potentials and signal transduction. Recent advances in optogenetic sensors open exciting new possibilities for cardiac research and allow us to answer research questions that cannot be addressed using the classic organic dyes. Especially thrilling is the possibility to use optogenetic sensors to record parameters of cardiac excitation and contraction in vivo. In addition, optogenetics provide a high spatial resolution, as sensors can be coupled to motifs and targeted to specific cell types and subcellular domains of the heart. In this review, we will give a comprehensive overview of relevant optogenetic sensors, how they can be utilised in cardiac research and how they have been applied in cardiac research up to now.
AB - Our current understanding of cardiac excitation and its coupling to contraction is largely based on ex vivo studies utilising fluorescent organic dyes to assess cardiac action potentials and signal transduction. Recent advances in optogenetic sensors open exciting new possibilities for cardiac research and allow us to answer research questions that cannot be addressed using the classic organic dyes. Especially thrilling is the possibility to use optogenetic sensors to record parameters of cardiac excitation and contraction in vivo. In addition, optogenetics provide a high spatial resolution, as sensors can be coupled to motifs and targeted to specific cell types and subcellular domains of the heart. In this review, we will give a comprehensive overview of relevant optogenetic sensors, how they can be utilised in cardiac research and how they have been applied in cardiac research up to now.
KW - Calcium cycling/excitation–contraction coupling
KW - Cell signalling/signal transduction
KW - Ion channels/membrane transport
KW - Physiology
UR - http://www.scopus.com/inward/record.url?scp=85028635000&partnerID=8YFLogxK
U2 - 10.1007/s00395-017-0645-y
DO - 10.1007/s00395-017-0645-y
M3 - Review article
C2 - 28861604
AN - SCOPUS:85028635000
SN - 0300-8428
VL - 112
JO - Basic Research in Cardiology
JF - Basic Research in Cardiology
IS - 5
M1 - 56
ER -