Canine Il-2: Characterization and optimal conditions for production

A. J J M Daemen; W. A. Buurman; C. J V D Linden; G. Groenewegen; G. Kootstra

doi:10.1016/0165-2427(84)90038-2

Canine Il-2: Characterization and optimal conditions for production

A. J J M Daemen^*, W. A. Buurman, C. J V D Linden, G. Groenewegen, G. Kootstra

^*Corresponding author for this work

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Abstract

The culture requirements for the production of canine Il-2 are reported. Several parameters have been tested, such as concentration of lectin, length of culture period and presence of the additives serum, polyethylene glycol (PEG) and phorbol myristic acetate (PMA). Optimal results have been obtained by stimulation of peripheral blood leukocytes with the lectin PHA (8 μg/ml) for 48 h. Techniques for the production of Il-2 containing supernatant free of PHA have been evaluated. Gel filtration chromatography of culture supernatant revealed that canine Il-2 has a molecular weight (m.w.) of approximately 30,000 daltons, similar to the m.w. of Il-2 produced by murine T cells.

Original language	English
Pages (from-to)	247-258
Number of pages	12
Journal	Veterinary Immunology and Immunopathology
Volume	5
Issue number	3
DOIs	https://doi.org/10.1016/0165-2427(84)90038-2
Publication status	Published - 1 Jan 1984

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title = "Canine Il-2: Characterization and optimal conditions for production",

abstract = "The culture requirements for the production of canine Il-2 are reported. Several parameters have been tested, such as concentration of lectin, length of culture period and presence of the additives serum, polyethylene glycol (PEG) and phorbol myristic acetate (PMA). Optimal results have been obtained by stimulation of peripheral blood leukocytes with the lectin PHA (8 μg/ml) for 48 h. Techniques for the production of Il-2 containing supernatant free of PHA have been evaluated. Gel filtration chromatography of culture supernatant revealed that canine Il-2 has a molecular weight (m.w.) of approximately 30,000 daltons, similar to the m.w. of Il-2 produced by murine T cells.",

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AU - Kootstra, G.

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Canine Il-2: Characterization and optimal conditions for production

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