TY - JOUR
T1 - Campylobacter DNA is present in circulating myelomonocytic cells of healthy persons and in persons with Guillain-Barré syndrome
AU - Van Rhijn, Ildiko
AU - Bleumink-Pluym, Nancy M.C.
AU - Van Putten, Jos P.M.
AU - Van den Berg, Leonard H.
N1 - Funding Information:
Received 24 July 2001; revised 20 September 2001; electronically published 3 January 2002. Presented in part: 11th International Workshop on Campylobacter, Helicobacter, and Related Organisms, Freiburg, Germany, September 2001 (abstract N18). The University Medical Center Utrecht medical ethics committee approved the study protocol. Financial support: Het Prinses Beatrix Fonds (support to I.V.R.). Reprints or correspondence: Dr. Ildiko Van Rhijn, UMC Utrecht, Dept. of Neurology, Rm. G02.320, PO Box 85500, 3508 GA Utrecht, The Netherlands ([email protected]).
PY - 2002/1/15
Y1 - 2002/1/15
N2 - Campylobacter jejuni is the prime cause of foodborne bacterial gastroenteritis. An important complication of C. jejuni enteritis is Guillain-Barré syndrome (GBS), an immune-mediated disorder of the peripheral nerve. The presence of C. jejuni DNA in peripheral blood mononuclear cells (PBMC) of patients with GBS, patients with C. jejuni enteritis, and healthy subjects was studied. Two target genes, the flagellin and the ceuE genes, were used for polymerase chain reaction (PCR) identification of Campylobacter species in DNA extracted from PBMC. Approximately 30% of the healthy subjects and 50% of the patients with GBS had PBMC containing C. jejuni DNA as verified by Southern blot analysis or sequencing of the PCR products. Cell sorting revealed that Campylobacter DNA was present in CD14+ and CD33+ populations, indicating that cells from the myelomonocytic lineage are the Campylobacter DNA-carrying cells. These findings show that Campylobacter DNA is present in blood cells of healthy humans, although viable bacteria could not be demonstrated.
AB - Campylobacter jejuni is the prime cause of foodborne bacterial gastroenteritis. An important complication of C. jejuni enteritis is Guillain-Barré syndrome (GBS), an immune-mediated disorder of the peripheral nerve. The presence of C. jejuni DNA in peripheral blood mononuclear cells (PBMC) of patients with GBS, patients with C. jejuni enteritis, and healthy subjects was studied. Two target genes, the flagellin and the ceuE genes, were used for polymerase chain reaction (PCR) identification of Campylobacter species in DNA extracted from PBMC. Approximately 30% of the healthy subjects and 50% of the patients with GBS had PBMC containing C. jejuni DNA as verified by Southern blot analysis or sequencing of the PCR products. Cell sorting revealed that Campylobacter DNA was present in CD14+ and CD33+ populations, indicating that cells from the myelomonocytic lineage are the Campylobacter DNA-carrying cells. These findings show that Campylobacter DNA is present in blood cells of healthy humans, although viable bacteria could not be demonstrated.
KW - Econometric and Statistical Methods: General
KW - Geneeskunde (GENK)
KW - Geneeskunde(GENK)
KW - Medical sciences
KW - Bescherming en bevordering van de menselijke gezondheid
UR - http://www.scopus.com/inward/record.url?scp=0037080383&partnerID=8YFLogxK
U2 - 10.1086/338264
DO - 10.1086/338264
M3 - Article
C2 - 11807702
SN - 0022-1899
VL - 185
SP - 262
EP - 265
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -