TY - JOUR
T1 - Breaking the resolution limits of 3D bioprinting
T2 - future opportunities and present challenges
AU - Zandrini, Tommaso
AU - Florczak, Sammy
AU - Levato, Riccardo
AU - Ovsianikov, Aleksandr
N1 - Funding Information:
R.L. acknowledges funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 949806, VOLUME-BIO) and from the European's Union's Horizon 2020 research and innovation program under grant agreement No 964497 (ENLIGHT). A.O. acknowledges funding from the European Research Council (ERC) (Grant agreement numbers 307701, LeBMEC and 772464, THIRST). The authors would like to thank Professor V. Mironov for fruitful discussions. A.O. is also a Co-Founder and CSO of UpNano GmbH, a recent spin-off of the TU Wien aiming at commercialization of MPL.
Funding Information:
R.L. acknowledges funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 949806 , VOLUME-BIO) and from the European's Union's Horizon 2020 research and innovation program under grant agreement No 964497 (ENLIGHT). A.O. acknowledges funding from the European Research Council (ERC) (Grant agreement numbers 307701 , LeBMEC and 772464 , THIRST). The authors would like to thank Professor V. Mironov for fruitful discussions.
Publisher Copyright:
© 2022
PY - 2023/5
Y1 - 2023/5
N2 - Bioprinting aims to produce 3D structures from which embedded cells can receive mechanical and chemical stimuli that influence their behavior, direct their organization and migration, and promote differentiation, in a similar way to what happens within the native extracellular matrix. However, limited spatial resolution has been a bottleneck for conventional 3D bioprinting approaches. Reproducing fine features at the cellular scale, while maintaining a reasonable printing volume, is necessary to enable the biofabrication of more complex and functional tissue and organ models. In this opinion article we recount the emergence of, and discuss the most promising, high-definition (HD) bioprinting techniques to achieve this goal, discussing which obstacles remain to be overcome, and which applications are envisioned in the tissue engineering field.
AB - Bioprinting aims to produce 3D structures from which embedded cells can receive mechanical and chemical stimuli that influence their behavior, direct their organization and migration, and promote differentiation, in a similar way to what happens within the native extracellular matrix. However, limited spatial resolution has been a bottleneck for conventional 3D bioprinting approaches. Reproducing fine features at the cellular scale, while maintaining a reasonable printing volume, is necessary to enable the biofabrication of more complex and functional tissue and organ models. In this opinion article we recount the emergence of, and discuss the most promising, high-definition (HD) bioprinting techniques to achieve this goal, discussing which obstacles remain to be overcome, and which applications are envisioned in the tissue engineering field.
KW - biofabrication
KW - cell electrowriting
KW - digital light processing
KW - HD bioprinting
KW - multiphoton lithography
KW - organ-on-a-chip
KW - volumeter printing
UR - http://www.scopus.com/inward/record.url?scp=85145290086&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2022.10.009
DO - 10.1016/j.tibtech.2022.10.009
M3 - Review article
C2 - 36513545
AN - SCOPUS:85145290086
SN - 0167-7799
VL - 41
SP - 604
EP - 614
JO - Trends in biotechnology
JF - Trends in biotechnology
IS - 5
ER -