Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device

A.H. Bruggink; N. de Jonge; M.F.M. van Oosterhout; D.F. van Wichen; E. de Koning; J.R. Lahpor; J.T.J.H. Kemperman; F.H.J. Gmelig Meyling; R.A. de Weger

doi:10.1016/j.helaun.2005.09.007

Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device

A.H. Bruggink, N. de Jonge, M.F.M. van Oosterhout, D.F. van Wichen, E. de Koning, J.R. Lahpor, J.T.J.H. Kemperman, F.H.J. Gmelig Meyling, R.A. de Weger

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background: Brain natriuretic peptide (BNP) is a cardiac neurohormone synthesized in cardiac ventricles. as a result of increased wall stress. Left ventricular assist device (LVAD) support in patients with end-stage heart failure results in reduced wall stress and therefore may change BNP levels in the heart.

Methods: BNP plasma levels were measured in 17 patients with end-stage HF before LVAD implantation and at 1 week, 1 month, and 3 months after LVAD support. BNP-messenger RNA (mRNA) expression in cardiac biopsy specimens of 27 patients before and after LVAD support was determined by quantitative polymerase chain reaction. Immunohistochemistry (IHC) and IHC-double staining was used in biopsy specimens from 32 patients before and after LVAD support to localize the BNP protein expression in the heart.

Results: BNP plasma levels significantly decreased from 1,872 +/- 1,098 pg/ml before implantation to 117 +/- 91 pg/ml at 3 months after LVAD implantation. This decrease in plasma levels was accompanied by a significant decrease in mRNA expression (relative quantity) in the heart. IHC and IHC-double staining showed BNP immunoreactivity in the cardiomyocytes, endothelial cells, infiltrating T cells, and macrophages.

Conclusions: The significant decrease in serum BNP concentration after LVAD support coincides with a decrease in BNP mRNA and protein expression in the heart. BNP is produced in the left ventricle not only by cardiomyocytes but also by endothelial cells, T cells, and macrophages. Unloading of the left ventricle by 4 LVAD results in decreased BNP expression in the heart and plasma and may play an important role in the reverse remodeling process of the heart.

Original language	English
Pages (from-to)	174-180
Number of pages	7
Journal	Journal of Heart and Lung Transplantation
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/j.helaun.2005.09.007
Publication status	Published - Feb 2006

Keywords

CARDIAC ALLOGRAFT-REJECTION
ACUTE MYOCARDIAL-INFARCTION
MATRIX METALLOPROTEINASES
DYSFUNCTION
EXPRESSION
RECOVERY
MARKER
IMPLANTATION
HYPERTROPHY
ACTIVATION

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Bruggink, A. H., de Jonge, N., van Oosterhout, M. F. M., van Wichen, D. F., de Koning, E., Lahpor, J. R., Kemperman, J. T. J. H., Gmelig Meyling, F. H. J., & de Weger, R. A. (2006). Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device. Journal of Heart and Lung Transplantation, 25(2), 174-180. https://doi.org/10.1016/j.helaun.2005.09.007

@article{34a297f5fbbc410694a7955cadaffcea,

title = "Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device",

abstract = "Background: Brain natriuretic peptide (BNP) is a cardiac neurohormone synthesized in cardiac ventricles. as a result of increased wall stress. Left ventricular assist device (LVAD) support in patients with end-stage heart failure results in reduced wall stress and therefore may change BNP levels in the heart.Methods: BNP plasma levels were measured in 17 patients with end-stage HF before LVAD implantation and at 1 week, 1 month, and 3 months after LVAD support. BNP-messenger RNA (mRNA) expression in cardiac biopsy specimens of 27 patients before and after LVAD support was determined by quantitative polymerase chain reaction. Immunohistochemistry (IHC) and IHC-double staining was used in biopsy specimens from 32 patients before and after LVAD support to localize the BNP protein expression in the heart.Results: BNP plasma levels significantly decreased from 1,872 +/- 1,098 pg/ml before implantation to 117 +/- 91 pg/ml at 3 months after LVAD implantation. This decrease in plasma levels was accompanied by a significant decrease in mRNA expression (relative quantity) in the heart. IHC and IHC-double staining showed BNP immunoreactivity in the cardiomyocytes, endothelial cells, infiltrating T cells, and macrophages.Conclusions: The significant decrease in serum BNP concentration after LVAD support coincides with a decrease in BNP mRNA and protein expression in the heart. BNP is produced in the left ventricle not only by cardiomyocytes but also by endothelial cells, T cells, and macrophages. Unloading of the left ventricle by 4 LVAD results in decreased BNP expression in the heart and plasma and may play an important role in the reverse remodeling process of the heart.",

keywords = "CARDIAC ALLOGRAFT-REJECTION, ACUTE MYOCARDIAL-INFARCTION, MATRIX METALLOPROTEINASES, DYSFUNCTION, EXPRESSION, RECOVERY, MARKER, IMPLANTATION, HYPERTROPHY, ACTIVATION",

author = "A.H. Bruggink and {de Jonge}, N. and {van Oosterhout}, M.F.M. and {van Wichen}, D.F. and {de Koning}, E. and J.R. Lahpor and J.T.J.H. Kemperman and {Gmelig Meyling}, F.H.J. and {de Weger}, R.A.",

year = "2006",

month = feb,

doi = "10.1016/j.helaun.2005.09.007",

language = "English",

volume = "25",

pages = "174--180",

journal = "Journal of Heart and Lung Transplantation",

issn = "1053-2498",

publisher = "Elsevier",

number = "2",

}

Bruggink, AH, de Jonge, N, van Oosterhout, MFM, van Wichen, DF, de Koning, E, Lahpor, JR, Kemperman, JTJH, Gmelig Meyling, FHJ & de Weger, RA 2006, 'Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device', Journal of Heart and Lung Transplantation, vol. 25, no. 2, pp. 174-180. https://doi.org/10.1016/j.helaun.2005.09.007

Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device. / Bruggink, A.H.; de Jonge, N.; van Oosterhout, M.F.M. et al.
In: Journal of Heart and Lung Transplantation, Vol. 25, No. 2, 02.2006, p. 174-180.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device

AU - Bruggink, A.H.

AU - de Jonge, N.

AU - van Oosterhout, M.F.M.

AU - van Wichen, D.F.

AU - de Koning, E.

AU - Lahpor, J.R.

AU - Kemperman, J.T.J.H.

AU - Gmelig Meyling, F.H.J.

AU - de Weger, R.A.

PY - 2006/2

Y1 - 2006/2

N2 - Background: Brain natriuretic peptide (BNP) is a cardiac neurohormone synthesized in cardiac ventricles. as a result of increased wall stress. Left ventricular assist device (LVAD) support in patients with end-stage heart failure results in reduced wall stress and therefore may change BNP levels in the heart.Methods: BNP plasma levels were measured in 17 patients with end-stage HF before LVAD implantation and at 1 week, 1 month, and 3 months after LVAD support. BNP-messenger RNA (mRNA) expression in cardiac biopsy specimens of 27 patients before and after LVAD support was determined by quantitative polymerase chain reaction. Immunohistochemistry (IHC) and IHC-double staining was used in biopsy specimens from 32 patients before and after LVAD support to localize the BNP protein expression in the heart.Results: BNP plasma levels significantly decreased from 1,872 +/- 1,098 pg/ml before implantation to 117 +/- 91 pg/ml at 3 months after LVAD implantation. This decrease in plasma levels was accompanied by a significant decrease in mRNA expression (relative quantity) in the heart. IHC and IHC-double staining showed BNP immunoreactivity in the cardiomyocytes, endothelial cells, infiltrating T cells, and macrophages.Conclusions: The significant decrease in serum BNP concentration after LVAD support coincides with a decrease in BNP mRNA and protein expression in the heart. BNP is produced in the left ventricle not only by cardiomyocytes but also by endothelial cells, T cells, and macrophages. Unloading of the left ventricle by 4 LVAD results in decreased BNP expression in the heart and plasma and may play an important role in the reverse remodeling process of the heart.

AB - Background: Brain natriuretic peptide (BNP) is a cardiac neurohormone synthesized in cardiac ventricles. as a result of increased wall stress. Left ventricular assist device (LVAD) support in patients with end-stage heart failure results in reduced wall stress and therefore may change BNP levels in the heart.Methods: BNP plasma levels were measured in 17 patients with end-stage HF before LVAD implantation and at 1 week, 1 month, and 3 months after LVAD support. BNP-messenger RNA (mRNA) expression in cardiac biopsy specimens of 27 patients before and after LVAD support was determined by quantitative polymerase chain reaction. Immunohistochemistry (IHC) and IHC-double staining was used in biopsy specimens from 32 patients before and after LVAD support to localize the BNP protein expression in the heart.Results: BNP plasma levels significantly decreased from 1,872 +/- 1,098 pg/ml before implantation to 117 +/- 91 pg/ml at 3 months after LVAD implantation. This decrease in plasma levels was accompanied by a significant decrease in mRNA expression (relative quantity) in the heart. IHC and IHC-double staining showed BNP immunoreactivity in the cardiomyocytes, endothelial cells, infiltrating T cells, and macrophages.Conclusions: The significant decrease in serum BNP concentration after LVAD support coincides with a decrease in BNP mRNA and protein expression in the heart. BNP is produced in the left ventricle not only by cardiomyocytes but also by endothelial cells, T cells, and macrophages. Unloading of the left ventricle by 4 LVAD results in decreased BNP expression in the heart and plasma and may play an important role in the reverse remodeling process of the heart.

KW - CARDIAC ALLOGRAFT-REJECTION

KW - ACUTE MYOCARDIAL-INFARCTION

KW - MATRIX METALLOPROTEINASES

KW - DYSFUNCTION

KW - EXPRESSION

KW - RECOVERY

KW - MARKER

KW - IMPLANTATION

KW - HYPERTROPHY

KW - ACTIVATION

U2 - 10.1016/j.helaun.2005.09.007

DO - 10.1016/j.helaun.2005.09.007

M3 - Article

SN - 1053-2498

VL - 25

SP - 174

EP - 180

JO - Journal of Heart and Lung Transplantation

JF - Journal of Heart and Lung Transplantation

IS - 2

ER -

Bruggink AH, de Jonge N, van Oosterhout MFM, van Wichen DF, de Koning E, Lahpor JR et al. Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device. Journal of Heart and Lung Transplantation. 2006 Feb;25(2):174-180. doi: 10.1016/j.helaun.2005.09.007

Brain natriuretic peptide is produced both by cardiornyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device

Abstract

Keywords

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