Brain natriuretic peptide is produced both by cardiomyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device

A.H. Bruggink; Nicolaas de Jonge; Matthijs F M van Oosterhout; Dick F Van Wichen; Erica de Koning; Jaap R Lahpor; Hans Kemperman; Frits H J Gmelig-Meyling; Roel A de Weger

doi:10.1016/j.healun.2005.09.007

Brain natriuretic peptide is produced both by cardiomyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device

A.H. Bruggink, Nicolaas de Jonge, Matthijs F M van Oosterhout, Dick F Van Wichen, Erica de Koning, Jaap R Lahpor, Hans Kemperman, Frits H J Gmelig-Meyling, Roel A de Weger

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Brain natriuretic peptide (BNP) is a cardiac neurohormone synthesized in cardiac ventricles as a result of increased wall stress. Left ventricular assist device (LVAD) support in patients with end-stage heart failure results in reduced wall stress and therefore may change BNP levels in the heart.

METHODS: BNP plasma levels were measured in 17 patients with end-stage HF before LVAD implantation and at 1 week, 1 month, and 3 months after LVAD support. BNP-messenger RNA (mRNA) expression in cardiac biopsy specimens of 27 patients before and after LVAD support was determined by quantitative polymerase chain reaction. Immunohistochemistry (IHC) and IHC-double staining was used in biopsy specimens from 32 patients before and after LVAD support to localize the BNP protein expression in the heart.

RESULTS: BNP plasma levels significantly decreased from 1,872 +/- 1,098 pg/ml before implantation to 117 +/- 91 pg/ml at 3 months after LVAD implantation. This decrease in plasma levels was accompanied by a significant decrease in mRNA expression (relative quantity) in the heart. IHC and IHC-double staining showed BNP immunoreactivity in the cardiomyocytes, endothelial cells, infiltrating T cells, and macrophages.

CONCLUSIONS: The significant decrease in serum BNP concentration after LVAD support coincides with a decrease in BNP mRNA and protein expression in the heart. BNP is produced in the left ventricle not only by cardiomyocytes but also by endothelial cells, T cells, and macrophages. Unloading of the left ventricle by a LVAD results in decreased BNP expression in the heart and plasma and may play an important role in the reverse remodeling process of the heart.

Original language	English
Pages (from-to)	174-80
Number of pages	7
Journal	Journal of Heart and Lung Transplantation
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/j.healun.2005.09.007
Publication status	Published - Feb 2006

Keywords

Adult
Antigens, CD45
Biopsy
Endothelium, Vascular
Female
Gene Expression Regulation
Heart Failure
Heart Ventricles
Heart-Assist Devices
Humans
Immunohistochemistry
Macrophages
Male
Middle Aged
Myocardium
Myocytes, Cardiac
Natriuretic Peptide, Brain
Polymerase Chain Reaction
RNA, Messenger
T-Lymphocytes
Tumor Necrosis Factor-alpha
Ventricular Remodeling

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10.1016/j.healun.2005.09.007

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Bruggink, A. H., de Jonge, N., van Oosterhout, M. F. M., Van Wichen, D. F., de Koning, E., Lahpor, J. R., Kemperman, H., Gmelig-Meyling, F. H. J., & de Weger, R. A. (2006). Brain natriuretic peptide is produced both by cardiomyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device. Journal of Heart and Lung Transplantation, 25(2), 174-80. https://doi.org/10.1016/j.healun.2005.09.007

@article{5673379e69ec4a4bada7d8f6d1620da6,

title = "Brain natriuretic peptide is produced both by cardiomyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device",

abstract = "BACKGROUND: Brain natriuretic peptide (BNP) is a cardiac neurohormone synthesized in cardiac ventricles as a result of increased wall stress. Left ventricular assist device (LVAD) support in patients with end-stage heart failure results in reduced wall stress and therefore may change BNP levels in the heart.METHODS: BNP plasma levels were measured in 17 patients with end-stage HF before LVAD implantation and at 1 week, 1 month, and 3 months after LVAD support. BNP-messenger RNA (mRNA) expression in cardiac biopsy specimens of 27 patients before and after LVAD support was determined by quantitative polymerase chain reaction. Immunohistochemistry (IHC) and IHC-double staining was used in biopsy specimens from 32 patients before and after LVAD support to localize the BNP protein expression in the heart.RESULTS: BNP plasma levels significantly decreased from 1,872 +/- 1,098 pg/ml before implantation to 117 +/- 91 pg/ml at 3 months after LVAD implantation. This decrease in plasma levels was accompanied by a significant decrease in mRNA expression (relative quantity) in the heart. IHC and IHC-double staining showed BNP immunoreactivity in the cardiomyocytes, endothelial cells, infiltrating T cells, and macrophages.CONCLUSIONS: The significant decrease in serum BNP concentration after LVAD support coincides with a decrease in BNP mRNA and protein expression in the heart. BNP is produced in the left ventricle not only by cardiomyocytes but also by endothelial cells, T cells, and macrophages. Unloading of the left ventricle by a LVAD results in decreased BNP expression in the heart and plasma and may play an important role in the reverse remodeling process of the heart.",

keywords = "Adult, Antigens, CD45, Biopsy, Endothelium, Vascular, Female, Gene Expression Regulation, Heart Failure, Heart Ventricles, Heart-Assist Devices, Humans, Immunohistochemistry, Macrophages, Male, Middle Aged, Myocardium, Myocytes, Cardiac, Natriuretic Peptide, Brain, Polymerase Chain Reaction, RNA, Messenger, T-Lymphocytes, Tumor Necrosis Factor-alpha, Ventricular Remodeling",

author = "A.H. Bruggink and {de Jonge}, Nicolaas and {van Oosterhout}, {Matthijs F M} and {Van Wichen}, {Dick F} and {de Koning}, Erica and Lahpor, {Jaap R} and Hans Kemperman and Gmelig-Meyling, {Frits H J} and {de Weger}, {Roel A}",

year = "2006",

month = feb,

doi = "10.1016/j.healun.2005.09.007",

language = "English",

volume = "25",

pages = "174--80",

journal = "Journal of Heart and Lung Transplantation",

issn = "1053-2498",

publisher = "Elsevier USA",

number = "2",

}

Bruggink, AH, de Jonge, N, van Oosterhout, MFM, Van Wichen, DF, de Koning, E, Lahpor, JR, Kemperman, H, Gmelig-Meyling, FHJ & de Weger, RA 2006, 'Brain natriuretic peptide is produced both by cardiomyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device', Journal of Heart and Lung Transplantation, vol. 25, no. 2, pp. 174-80. https://doi.org/10.1016/j.healun.2005.09.007

Brain natriuretic peptide is produced both by cardiomyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device. / Bruggink, A.H.; de Jonge, Nicolaas; van Oosterhout, Matthijs F M et al.
In: Journal of Heart and Lung Transplantation, Vol. 25, No. 2, 02.2006, p. 174-80.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Brain natriuretic peptide is produced both by cardiomyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device

AU - Bruggink, A.H.

AU - de Jonge, Nicolaas

AU - van Oosterhout, Matthijs F M

AU - Van Wichen, Dick F

AU - de Koning, Erica

AU - Lahpor, Jaap R

AU - Kemperman, Hans

AU - Gmelig-Meyling, Frits H J

AU - de Weger, Roel A

PY - 2006/2

Y1 - 2006/2

N2 - BACKGROUND: Brain natriuretic peptide (BNP) is a cardiac neurohormone synthesized in cardiac ventricles as a result of increased wall stress. Left ventricular assist device (LVAD) support in patients with end-stage heart failure results in reduced wall stress and therefore may change BNP levels in the heart.METHODS: BNP plasma levels were measured in 17 patients with end-stage HF before LVAD implantation and at 1 week, 1 month, and 3 months after LVAD support. BNP-messenger RNA (mRNA) expression in cardiac biopsy specimens of 27 patients before and after LVAD support was determined by quantitative polymerase chain reaction. Immunohistochemistry (IHC) and IHC-double staining was used in biopsy specimens from 32 patients before and after LVAD support to localize the BNP protein expression in the heart.RESULTS: BNP plasma levels significantly decreased from 1,872 +/- 1,098 pg/ml before implantation to 117 +/- 91 pg/ml at 3 months after LVAD implantation. This decrease in plasma levels was accompanied by a significant decrease in mRNA expression (relative quantity) in the heart. IHC and IHC-double staining showed BNP immunoreactivity in the cardiomyocytes, endothelial cells, infiltrating T cells, and macrophages.CONCLUSIONS: The significant decrease in serum BNP concentration after LVAD support coincides with a decrease in BNP mRNA and protein expression in the heart. BNP is produced in the left ventricle not only by cardiomyocytes but also by endothelial cells, T cells, and macrophages. Unloading of the left ventricle by a LVAD results in decreased BNP expression in the heart and plasma and may play an important role in the reverse remodeling process of the heart.

AB - BACKGROUND: Brain natriuretic peptide (BNP) is a cardiac neurohormone synthesized in cardiac ventricles as a result of increased wall stress. Left ventricular assist device (LVAD) support in patients with end-stage heart failure results in reduced wall stress and therefore may change BNP levels in the heart.METHODS: BNP plasma levels were measured in 17 patients with end-stage HF before LVAD implantation and at 1 week, 1 month, and 3 months after LVAD support. BNP-messenger RNA (mRNA) expression in cardiac biopsy specimens of 27 patients before and after LVAD support was determined by quantitative polymerase chain reaction. Immunohistochemistry (IHC) and IHC-double staining was used in biopsy specimens from 32 patients before and after LVAD support to localize the BNP protein expression in the heart.RESULTS: BNP plasma levels significantly decreased from 1,872 +/- 1,098 pg/ml before implantation to 117 +/- 91 pg/ml at 3 months after LVAD implantation. This decrease in plasma levels was accompanied by a significant decrease in mRNA expression (relative quantity) in the heart. IHC and IHC-double staining showed BNP immunoreactivity in the cardiomyocytes, endothelial cells, infiltrating T cells, and macrophages.CONCLUSIONS: The significant decrease in serum BNP concentration after LVAD support coincides with a decrease in BNP mRNA and protein expression in the heart. BNP is produced in the left ventricle not only by cardiomyocytes but also by endothelial cells, T cells, and macrophages. Unloading of the left ventricle by a LVAD results in decreased BNP expression in the heart and plasma and may play an important role in the reverse remodeling process of the heart.

KW - Adult

KW - Antigens, CD45

KW - Biopsy

KW - Endothelium, Vascular

KW - Female

KW - Gene Expression Regulation

KW - Heart Failure

KW - Heart Ventricles

KW - Heart-Assist Devices

KW - Humans

KW - Immunohistochemistry

KW - Macrophages

KW - Male

KW - Middle Aged

KW - Myocardium

KW - Myocytes, Cardiac

KW - Natriuretic Peptide, Brain

KW - Polymerase Chain Reaction

KW - RNA, Messenger

KW - T-Lymphocytes

KW - Tumor Necrosis Factor-alpha

KW - Ventricular Remodeling

U2 - 10.1016/j.healun.2005.09.007

DO - 10.1016/j.healun.2005.09.007

M3 - Article

C2 - 16446217

SN - 1053-2498

VL - 25

SP - 174

EP - 180

JO - Journal of Heart and Lung Transplantation

JF - Journal of Heart and Lung Transplantation

IS - 2

ER -

Brain natriuretic peptide is produced both by cardiomyocytes and cells infiltrating the heart in patients with severe heart failure supported by a left ventricular assist device

Abstract

Keywords

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