Biomaterial-associated persistence of Streptococcus epidermidis in pericatheter macrophages

JJ Boelens*, J Dankert, JL Murk, JJ Weening, T van der Poll, KP Dingemans, L Koole, JD Laman, SAJ Zaat

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Biomaterial surfaces may be modified to reduce bacterial adhesion. The susceptibility in mice to Staphylococcus epidermidis infection in tissue surrounding the commonly used catheter materials-silicon elastomer (SE), polyamide (PA), and their surface-modified polyvinylpyrrolidone (PVP)-grafted derivatives, SE-PVP and PA-PVP, respectively-was assessed. Abscesses developed around SE-PVP Around SE, PA, and PA-PVP catheters, no signs of infection were observed, although mice carrying PA-PVP developed septicemia after 14-21 days. S. epidermidis was cultured from the tissue surrounding PA-PVP segments. Cells around PA-PVP segments containing large numbers of bacteria were identified as macrophages by use of immunohistochemistry and electron microscopy. This persistence of intracellular bacteria was also observed around SE-PVP, SE, and PA, catheters, although to a lesser extent. The cytokine profiles around the 4 materials were different. Implanted biomaterial induces an inflammatory response favorable to the persistence of S. epidermidis. Intracellular persistence of bacteria inside macrophages may be a pivotal process in the pathogenesis of biomaterial-associated infection.

Original languageEnglish
Pages (from-to)1337-1349
Number of pages13
JournalJournal of Infectious Diseases
Volume181
Issue number4
Publication statusPublished - Apr 2000
Event39th Interscience Conference on Antimicrobial Agents and Chemotherapy - SAN FRANCISCO, Netherlands
Duration: 26 Sept 199829 Sept 1998

Keywords

  • COAGULASE-NEGATIVE STAPHYLOCOCCI
  • FOREIGN-BODY INFECTIONS
  • TUMOR-NECROSIS-FACTOR
  • BIOMEDICAL POLYMERS
  • INTERFERON-GAMMA
  • INTERLEUKIN-10
  • ADHESION
  • MODEL
  • PATHOGENESIS
  • NEUTROPHILS

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