TY - JOUR
T1 - Binary IS typing for staphylococcus aureus
AU - Budding, Andries E.
AU - Vandenbroucke-Grauls, Christina M.J.E.
AU - Melles, Damian C.
AU - van Duijkeren, Engeline
AU - Kluytmans, Jan A.
AU - Savelkoul, Paul H.M.
PY - 2010
Y1 - 2010
N2 - Background: We present an easily applicable test for rapid binary typing of Staphylococcus aureus: binary interspace (IS) typing. This test is a further development of a previously described molecular typing technique that is based on length polymorphisms of the 16S-23S rDNA interspace region of S. aureus. Methodology/Principal Findings: A novel approach of IS-typing was performed in which binary profiles are created. 424 human and animal derived MRSA and MSSA isolates were tested and a subset of these isolates was compared with multi locus sequence typing (MLST) and Amplified Fragment Length Polymorphism (AFLP). Binary IS typing had a high discriminatory potential and a good correlation with MLST and AFLP. Conclusions/Significance: Binary IS typing is easy to perform and binary profiles can be generated in a standardized fashion. These two features, combined with the high correlation with MLST clonal complexes, make the technique applicable for large-scale inter-laboratory molecular epidemiological comparisons.
AB - Background: We present an easily applicable test for rapid binary typing of Staphylococcus aureus: binary interspace (IS) typing. This test is a further development of a previously described molecular typing technique that is based on length polymorphisms of the 16S-23S rDNA interspace region of S. aureus. Methodology/Principal Findings: A novel approach of IS-typing was performed in which binary profiles are created. 424 human and animal derived MRSA and MSSA isolates were tested and a subset of these isolates was compared with multi locus sequence typing (MLST) and Amplified Fragment Length Polymorphism (AFLP). Binary IS typing had a high discriminatory potential and a good correlation with MLST and AFLP. Conclusions/Significance: Binary IS typing is easy to perform and binary profiles can be generated in a standardized fashion. These two features, combined with the high correlation with MLST clonal complexes, make the technique applicable for large-scale inter-laboratory molecular epidemiological comparisons.
UR - https://www.scopus.com/pages/publications/78149462001
U2 - 10.1371/journal.pone.0013671
DO - 10.1371/journal.pone.0013671
M3 - Article
C2 - 21060683
AN - SCOPUS:78149462001
SN - 1932-6203
VL - 5
JO - PLoS ONE
JF - PLoS ONE
IS - 10
M1 - e13671
ER -