TY - JOUR
T1 - Beta2-adrenergic receptor haplotype and bronchodilator response to salbutamol in patients with acute exacerbations of COPD
AU - Mokry, Michal
AU - Joppa, Pavol
AU - Slaba, Eva
AU - Zidzik, Jozef
AU - Habalova, Viera
AU - Kluchova, Zuzana
AU - Micietova, Lydia
AU - Rozborilova, Eva
AU - Salagovic, Jan
AU - Tkacova, Ruzena
PY - 2008/8
Y1 - 2008/8
N2 - Background: The role of the beta2 (β2)- adrenergic receptor (ADRB2) genotype in patients with chronic obstructive pulmonary disease (COPD) is unclear. In patients with acute exacerbations of COPD (AECOPD), we assessed the role of ADRB2 haplotypes in morning lung function and in the bronchodilator response to salbutamol. Material/Methods: In 107 patients with AECOPD, polymorphisms in the amino acid position 16 (Arg16/Gly16) and 27 (Gln27/Glu27) of the ADRB 2 gene were assessed by allele-specific polymerase chain reaction, identifying 31 subjects with the Gly16/Glu27-negative and 76 with the Gly16/Glu27-positive ADRB2 haplotype. Pulmonary function and bronchodilator response to salbutamol were assessed using bodyplethysmography. Results: Forced expiratory volume in 1 second (FEV1) and peak expiratory flow (PEF) were significantly higher in the Gly16/Glu27-negative compared to the Gly16/Glu27-positive haplotype group at baseline (49.7±2.9% vs 42.4±1.8% predicted, P=0.037; 44.0±2.2% vs 36.4±1.6% predicted, P=0.008, respectively). FEV1, PEF, and forced vital capacity (FVC) increased from baseline to after salbutamol treatment in both the Gly16/Glu27-negative and the Gly16/Glu27-positive ADRB2 haplotype groups (P<0.001 for all comparisons). Values for FEV1 and PEF after administration of the bronchodilator were significantly higher in the Gly16/Glu27-negative haplotype group compared with the Gly16/Glu27-positive haplotype group (P=0.030 and P=0.034, respectively). No differences were observed in DeltaFEV1, DeltaPEF, or DeltaFVC after bronchodilation between the 2 ADRB2 haplotype groups (12.2±1.8% vs 14.5±1.5% predicted, P=0.393; 12.2±3.3% vs 20.8±3.2% predicted, P=0.117; 9.1±2.3% vs 10.4±1.9% predicted, P=0.707, respectively). Conclusions: The present findings suggest that the ADBR2 gene haplotypes may affect the severity of obstructive ventilatory impairment but not the immediate response to salbutamol during AECOPD.
AB - Background: The role of the beta2 (β2)- adrenergic receptor (ADRB2) genotype in patients with chronic obstructive pulmonary disease (COPD) is unclear. In patients with acute exacerbations of COPD (AECOPD), we assessed the role of ADRB2 haplotypes in morning lung function and in the bronchodilator response to salbutamol. Material/Methods: In 107 patients with AECOPD, polymorphisms in the amino acid position 16 (Arg16/Gly16) and 27 (Gln27/Glu27) of the ADRB 2 gene were assessed by allele-specific polymerase chain reaction, identifying 31 subjects with the Gly16/Glu27-negative and 76 with the Gly16/Glu27-positive ADRB2 haplotype. Pulmonary function and bronchodilator response to salbutamol were assessed using bodyplethysmography. Results: Forced expiratory volume in 1 second (FEV1) and peak expiratory flow (PEF) were significantly higher in the Gly16/Glu27-negative compared to the Gly16/Glu27-positive haplotype group at baseline (49.7±2.9% vs 42.4±1.8% predicted, P=0.037; 44.0±2.2% vs 36.4±1.6% predicted, P=0.008, respectively). FEV1, PEF, and forced vital capacity (FVC) increased from baseline to after salbutamol treatment in both the Gly16/Glu27-negative and the Gly16/Glu27-positive ADRB2 haplotype groups (P<0.001 for all comparisons). Values for FEV1 and PEF after administration of the bronchodilator were significantly higher in the Gly16/Glu27-negative haplotype group compared with the Gly16/Glu27-positive haplotype group (P=0.030 and P=0.034, respectively). No differences were observed in DeltaFEV1, DeltaPEF, or DeltaFVC after bronchodilation between the 2 ADRB2 haplotype groups (12.2±1.8% vs 14.5±1.5% predicted, P=0.393; 12.2±3.3% vs 20.8±3.2% predicted, P=0.117; 9.1±2.3% vs 10.4±1.9% predicted, P=0.707, respectively). Conclusions: The present findings suggest that the ADBR2 gene haplotypes may affect the severity of obstructive ventilatory impairment but not the immediate response to salbutamol during AECOPD.
KW - β-adrenoreceptor
KW - COPD
KW - Haplotype
KW - Salbutamol
UR - http://www.scopus.com/inward/record.url?scp=50249109629&partnerID=8YFLogxK
M3 - Article
C2 - 18667995
AN - SCOPUS:50249109629
SN - 1234-1010
VL - 14
SP - CR392-CR398
JO - Medical science monitor
JF - Medical science monitor
IS - 8
ER -