Best practice standards for circular RNA research

Anne F. Nielsen; Albrecht Bindereif; Irene Bozzoni; Mor Hanan; Thomas B. Hansen; Manuel Irimia; Sebastian Kadener; Lasse S. Kristensen; Ivano Legnini; Mariangela Morlando; Morten T. Jarlstad Olesen; R. Jeroen Pasterkamp; Stephan Preibisch; Nikolaus Rajewsky; Christin Suenkel; Jørgen Kjems

doi:10.1038/s41592-022-01487-2

Best practice standards for circular RNA research

Anne F. Nielsen
, Albrecht Bindereif
, Irene Bozzoni
, Mor Hanan
, Thomas B. Hansen
, Manuel Irimia
, Sebastian Kadener
, Lasse S. Kristensen
, Ivano Legnini
, Mariangela Morlando
, Morten T. Jarlstad Olesen
, R. Jeroen Pasterkamp
, Stephan Preibisch
, Nikolaus Rajewsky
, Christin Suenkel
, Jørgen Kjems^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

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Abstract

Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

Original language	English
Pages (from-to)	1208-1220
Number of pages	13
Journal	Nature Methods
Volume	19
Issue number	10
DOIs	https://doi.org/10.1038/s41592-022-01487-2
Publication status	Published - Oct 2022

Keywords

RNA Splicing
RNA, Circular
RNA/genetics

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10.1038/s41592-022-01487-2

s41592-022-01487-2Final published version, 1.57 MBLicence: Taverne

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Nielsen, A. F., Bindereif, A., Bozzoni, I., Hanan, M., Hansen, T. B., Irimia, M., Kadener, S., Kristensen, L. S., Legnini, I., Morlando, M., Jarlstad Olesen, M. T., Pasterkamp, R. J., Preibisch, S., Rajewsky, N., Suenkel, C., & Kjems, J. (2022). Best practice standards for circular RNA research. Nature Methods, 19(10), 1208-1220. https://doi.org/10.1038/s41592-022-01487-2

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title = "Best practice standards for circular RNA research",

abstract = "Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors{\textquoteright} experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.",

keywords = "RNA Splicing, RNA, Circular, RNA/genetics",

author = "Nielsen, \{Anne F.\} and Albrecht Bindereif and Irene Bozzoni and Mor Hanan and Hansen, \{Thomas B.\} and Manuel Irimia and Sebastian Kadener and Kristensen, \{Lasse S.\} and Ivano Legnini and Mariangela Morlando and \{Jarlstad Olesen\}, \{Morten T.\} and Pasterkamp, \{R. Jeroen\} and Stephan Preibisch and Nikolaus Rajewsky and Christin Suenkel and J{\o}rgen Kjems",

note = "Funding Information: This work was supported by the European Union{\textquoteright}s Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska-Curie grant agreement no. 721890 (circRTrain). A.F.N. and J.K. are funded by the Danish National Research Foundation grant no. 135 (CellPAT). M.T.J.O. is funded by the Danish Cancer society grant R269-A15768. L.S.K. is funded by the Lundbeck Foundation (R307-2018-3433). I.B. acknowledges funding from ERC-2019-SyG 855923-ASTRA and from AIRC-Progetto IG 2019 ID no. 23053. A.B. is funded by the Deutsche Forschungsgemeinschaft via grants RTG 2355, SPP 1935 and RU5116. S.K. acknowledges funding from the National Institute of Health (R01GM124406 and R01AG057700). Publisher Copyright: {\textcopyright} 2022, Springer Nature America, Inc.",

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month = oct,

doi = "10.1038/s41592-022-01487-2",

language = "English",

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T1 - Best practice standards for circular RNA research

AU - Nielsen, Anne F.

AU - Bindereif, Albrecht

AU - Bozzoni, Irene

AU - Hanan, Mor

AU - Hansen, Thomas B.

AU - Irimia, Manuel

AU - Kadener, Sebastian

AU - Kristensen, Lasse S.

AU - Legnini, Ivano

AU - Morlando, Mariangela

AU - Jarlstad Olesen, Morten T.

AU - Pasterkamp, R. Jeroen

AU - Preibisch, Stephan

AU - Rajewsky, Nikolaus

AU - Suenkel, Christin

AU - Kjems, Jørgen

N1 - Funding Information: This work was supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 721890 (circRTrain). A.F.N. and J.K. are funded by the Danish National Research Foundation grant no. 135 (CellPAT). M.T.J.O. is funded by the Danish Cancer society grant R269-A15768. L.S.K. is funded by the Lundbeck Foundation (R307-2018-3433). I.B. acknowledges funding from ERC-2019-SyG 855923-ASTRA and from AIRC-Progetto IG 2019 ID no. 23053. A.B. is funded by the Deutsche Forschungsgemeinschaft via grants RTG 2355, SPP 1935 and RU5116. S.K. acknowledges funding from the National Institute of Health (R01GM124406 and R01AG057700). Publisher Copyright: © 2022, Springer Nature America, Inc.

PY - 2022/10

Y1 - 2022/10

N2 - Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

AB - Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors’ experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

KW - RNA Splicing

KW - RNA, Circular

KW - RNA/genetics

UR - https://www.scopus.com/pages/publications/85130997875

U2 - 10.1038/s41592-022-01487-2

DO - 10.1038/s41592-022-01487-2

M3 - Review article

C2 - 35618955

AN - SCOPUS:85130997875

SN - 1548-7091

VL - 19

SP - 1208

EP - 1220

JO - Nature Methods

JF - Nature Methods

IS - 10

ER -

Best practice standards for circular RNA research

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