TY - JOUR
T1 - Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism
AU - Ouyang, Xudong
AU - Hoeksma, Jelmer
AU - van der Velden, Gisela
AU - Beenker, Wouter A G
AU - van Triest, Maria H
AU - Burgering, Boudewijn M T
AU - den Hertog, Jeroen
N1 - Funding Information:
We like to thank Albert Heck, Cees Versluis and Arjan Barendregt for their help with HRMS measurements, Geert-Jan Boons, Rob Liskamp, John Kruijtzer, Johan Kemmink and Justyna Dobruchowska for their help with NMR measurements, Ronnie Lubbers for critical reading of the manuscript and Yara Mangindaan, Helen Buttstedt and Harm van Es, students of the den Hertog Lab at the Hubrecht Institute for technical support. This project was supported by the Chinese Scholarship Council (CSC).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Antimicrobial resistance has become one of the major threats to human health. Therefore, there is a strong need for novel antimicrobials with new mechanisms of action. The kingdom of fungi is an excellent source of antimicrobials for this purpose because it encompasses countless fungal species that harbor unusual metabolic pathways. Previously, we have established a library of secondary metabolites from 10,207 strains of fungi. Here, we screened for antimicrobial activity of the library against seven pathogenic bacterial strains and investigated the identity of the active compounds using ethyl acetate extraction, activity-directed purification using HPLC fractionation and chemical analyses. We initially found 280 antimicrobial strains and subsequently identified 17 structurally distinct compounds from 26 strains upon further analysis. All but one of these compounds, berkchaetoazaphilone B (BAB), were known to have antimicrobial activity. Here, we studied the antimicrobial properties of BAB, and found that BAB affected energy metabolism in both prokaryotic and eukaryotic cells. We conclude that fungi are a rich source of chemically diverse secondary metabolites with antimicrobial activity.
AB - Antimicrobial resistance has become one of the major threats to human health. Therefore, there is a strong need for novel antimicrobials with new mechanisms of action. The kingdom of fungi is an excellent source of antimicrobials for this purpose because it encompasses countless fungal species that harbor unusual metabolic pathways. Previously, we have established a library of secondary metabolites from 10,207 strains of fungi. Here, we screened for antimicrobial activity of the library against seven pathogenic bacterial strains and investigated the identity of the active compounds using ethyl acetate extraction, activity-directed purification using HPLC fractionation and chemical analyses. We initially found 280 antimicrobial strains and subsequently identified 17 structurally distinct compounds from 26 strains upon further analysis. All but one of these compounds, berkchaetoazaphilone B (BAB), were known to have antimicrobial activity. Here, we studied the antimicrobial properties of BAB, and found that BAB affected energy metabolism in both prokaryotic and eukaryotic cells. We conclude that fungi are a rich source of chemically diverse secondary metabolites with antimicrobial activity.
UR - http://www.scopus.com/inward/record.url?scp=85115417884&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-98252-w
DO - 10.1038/s41598-021-98252-w
M3 - Article
C2 - 34548600
SN - 2045-2322
VL - 11
SP - 1
EP - 13
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 18774
ER -