Abstract
Silica high-performance liquid chromatographic separation of phospho- and sphingolipids of biological origin using a mobile phase containing phosphoric acid leads to gradual hydrolysis of plasmalogens during their passage through the column. The resulting 2-acyl lyso analogues give rise to peaks that tail in the direction of the parent intact plasmalogen. Tailing can be prevented by previous complete acid hydrolysis of plasmalogens. Direct high-performance liquid chromatographic profiling of phospholipids, their plasmalogens (as 2-acyl lyso analogues) and sphingolipids is probably the method of choice for the diagnosis of patients with deficient plasmalogen biosynthesis caused by peroxisomal abnormalities.
| Original language | English |
|---|---|
| Pages (from-to) | 411-6 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 434 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 30 Dec 1988 |
Keywords
- Chromatography, High Pressure Liquid
- Humans
- Indicators and Reagents
- Phospholipids/blood
- Phosphoric Acids
- Plasmalogens/blood
- Sphingolipids/blood
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