Behaviour of plasmalogens during high-performance liquid chromatography on a silica column with a mobile phase containing phosphoric acid

E B Hoving; J Prins; H M Rutgers; F A Muskiet

doi:10.1016/s0378-4347(88)80007-0

Behaviour of plasmalogens during high-performance liquid chromatography on a silica column with a mobile phase containing phosphoric acid

E B Hoving, J Prins, H M Rutgers, F A Muskiet

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Silica high-performance liquid chromatographic separation of phospho- and sphingolipids of biological origin using a mobile phase containing phosphoric acid leads to gradual hydrolysis of plasmalogens during their passage through the column. The resulting 2-acyl lyso analogues give rise to peaks that tail in the direction of the parent intact plasmalogen. Tailing can be prevented by previous complete acid hydrolysis of plasmalogens. Direct high-performance liquid chromatographic profiling of phospholipids, their plasmalogens (as 2-acyl lyso analogues) and sphingolipids is probably the method of choice for the diagnosis of patients with deficient plasmalogen biosynthesis caused by peroxisomal abnormalities.

Original language	English
Pages (from-to)	411-6
Number of pages	6
Journal	Journal of chromatography
Volume	434
Issue number	2
DOIs	https://doi.org/10.1016/s0378-4347(88)80007-0
Publication status	Published - 30 Dec 1988

Keywords

Chromatography, High Pressure Liquid
Humans
Indicators and Reagents
Phospholipids/blood
Phosphoric Acids
Plasmalogens/blood
Sphingolipids/blood

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10.1016/s0378-4347(88)80007-0

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title = "Behaviour of plasmalogens during high-performance liquid chromatography on a silica column with a mobile phase containing phosphoric acid",

abstract = "Silica high-performance liquid chromatographic separation of phospho- and sphingolipids of biological origin using a mobile phase containing phosphoric acid leads to gradual hydrolysis of plasmalogens during their passage through the column. The resulting 2-acyl lyso analogues give rise to peaks that tail in the direction of the parent intact plasmalogen. Tailing can be prevented by previous complete acid hydrolysis of plasmalogens. Direct high-performance liquid chromatographic profiling of phospholipids, their plasmalogens (as 2-acyl lyso analogues) and sphingolipids is probably the method of choice for the diagnosis of patients with deficient plasmalogen biosynthesis caused by peroxisomal abnormalities.",

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T1 - Behaviour of plasmalogens during high-performance liquid chromatography on a silica column with a mobile phase containing phosphoric acid

AU - Hoving, E B

AU - Prins, J

AU - Rutgers, H M

AU - Muskiet, F A

PY - 1988/12/30

Y1 - 1988/12/30

N2 - Silica high-performance liquid chromatographic separation of phospho- and sphingolipids of biological origin using a mobile phase containing phosphoric acid leads to gradual hydrolysis of plasmalogens during their passage through the column. The resulting 2-acyl lyso analogues give rise to peaks that tail in the direction of the parent intact plasmalogen. Tailing can be prevented by previous complete acid hydrolysis of plasmalogens. Direct high-performance liquid chromatographic profiling of phospholipids, their plasmalogens (as 2-acyl lyso analogues) and sphingolipids is probably the method of choice for the diagnosis of patients with deficient plasmalogen biosynthesis caused by peroxisomal abnormalities.

AB - Silica high-performance liquid chromatographic separation of phospho- and sphingolipids of biological origin using a mobile phase containing phosphoric acid leads to gradual hydrolysis of plasmalogens during their passage through the column. The resulting 2-acyl lyso analogues give rise to peaks that tail in the direction of the parent intact plasmalogen. Tailing can be prevented by previous complete acid hydrolysis of plasmalogens. Direct high-performance liquid chromatographic profiling of phospholipids, their plasmalogens (as 2-acyl lyso analogues) and sphingolipids is probably the method of choice for the diagnosis of patients with deficient plasmalogen biosynthesis caused by peroxisomal abnormalities.

KW - Chromatography, High Pressure Liquid

KW - Humans

KW - Indicators and Reagents

KW - Phospholipids/blood

KW - Phosphoric Acids

KW - Plasmalogens/blood

KW - Sphingolipids/blood

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DO - 10.1016/s0378-4347(88)80007-0

M3 - Article

C2 - 3246530

VL - 434

SP - 411

EP - 416

JO - Journal of chromatography

JF - Journal of chromatography

IS - 2

ER -

Behaviour of plasmalogens during high-performance liquid chromatography on a silica column with a mobile phase containing phosphoric acid

Abstract

Keywords

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