Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection

Mark H T Stappers; Ferry Hagen; Peter Reimnitz; Johan W Mouton; Jacques F Meis; Inge C Gyssens

doi:10.1016/j.diagmicrobio.2015.12.017

Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection

Mark H T Stappers
, Ferry Hagen
, Peter Reimnitz
, Johan W Mouton
, Jacques F Meis
, Inge C Gyssens^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples.

Original language	English
Pages (from-to)	263-5
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	85
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2015.12.017
Publication status	Published - Jun 2016
Externally published	Yes

Keywords

Abscess/microbiology
Bacteriological Techniques/methods
Bacteroides Infections/diagnosis
Bacteroides fragilis/isolation & purification
Biopsy
Diabetic Foot/microbiology
Humans
Molecular Diagnostic Techniques/methods
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity

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10.1016/j.diagmicrobio.2015.12.017

Cite this

@article{cbbace3516934e93a4d302209c7ca537,

title = "Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection",

abstract = "Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12\% for B. fragilis and could improve the detection of B. fragilis in clinical samples.",

keywords = "Abscess/microbiology, Bacteriological Techniques/methods, Bacteroides Infections/diagnosis, Bacteroides fragilis/isolation \& purification, Biopsy, Diabetic Foot/microbiology, Humans, Molecular Diagnostic Techniques/methods, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity",

author = "Stappers, \{Mark H T\} and Ferry Hagen and Peter Reimnitz and Mouton, \{Johan W\} and Meis, \{Jacques F\} and Gyssens, \{Inge C\}",

year = "2016",

month = jun,

doi = "10.1016/j.diagmicrobio.2015.12.017",

language = "English",

volume = "85",

pages = "263--5",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier",

number = "2",

}

Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection. / Stappers, Mark H T; Hagen, Ferry; Reimnitz, Peter et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 85, No. 2, 06.2016, p. 263-5.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections

T2 - direct molecular versus culture-based detection

AU - Stappers, Mark H T

AU - Hagen, Ferry

AU - Reimnitz, Peter

AU - Mouton, Johan W

AU - Meis, Jacques F

AU - Gyssens, Inge C

PY - 2016/6

Y1 - 2016/6

N2 - Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples.

AB - Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples.

KW - Abscess/microbiology

KW - Bacteriological Techniques/methods

KW - Bacteroides Infections/diagnosis

KW - Bacteroides fragilis/isolation & purification

KW - Biopsy

KW - Diabetic Foot/microbiology

KW - Humans

KW - Molecular Diagnostic Techniques/methods

KW - Real-Time Polymerase Chain Reaction

KW - Sensitivity and Specificity

U2 - 10.1016/j.diagmicrobio.2015.12.017

DO - 10.1016/j.diagmicrobio.2015.12.017

M3 - Article

C2 - 27112830

SN - 0732-8893

VL - 85

SP - 263

EP - 265

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

ER -

Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection

Abstract

Keywords

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