Automation of a phospho-STAT5 staining procedure for flow cytometry for application in drug discovery

Fabrice Malergue, Andreas van Agthoven, Caroline Scifo, Dave Egan, Ger J Strous

Research output: Contribution to journalArticleAcademicpeer-review


Drug discovery often requires the screening of compound libraries on tissue cultured cells. Some major targets in drug discovery belong to signal transduction pathways, and PerFix EXPOSE* allows easy flow cytometry phospho assays. We thus investigated the possibility to further simplify and automate this assay, to allow the direct screening of drugs targeting signaling pathways. We show here the sensitivity of this fully automated assay on human growth hormone (hGH)-driven JAK/STAT5-activated IM-9 cells, and we discuss the throughput of this system, which is compatible with medium-throughput drug screening. Because the kit works directly on whole blood samples, ex-vivo assays are also possible with this approach, which could allow for the screening of drugs under more physiological conditions.

Original languageEnglish
Pages (from-to)416-21
Number of pages6
JournalJournal of biomolecular screening
Issue number3
Publication statusPublished - 2015


  • Automation
  • Cell Line
  • Drug Discovery
  • Drug Evaluation, Preclinical
  • Flow Cytometry
  • High-Throughput Screening Assays
  • Human Growth Hormone
  • Humans
  • Janus Kinases
  • Permeability
  • Phosphorylation
  • Reproducibility of Results
  • STAT5 Transcription Factor
  • Small Molecule Libraries


Dive into the research topics of 'Automation of a phospho-STAT5 staining procedure for flow cytometry for application in drug discovery'. Together they form a unique fingerprint.

Cite this