TY - JOUR
T1 - Arachidonic acid can induce leukotriene C4 formation by purified human eosinophils in the absence of other stimuli
AU - Kok, Paul T.M.
AU - Hamelink, Maartje L.
AU - Kijne, Arda M.
AU - Verhagen, Jan
AU - Koenderman, Leo
AU - Bruynzeel, Pieter L.B.
PY - 1988/6/16
Y1 - 1988/6/16
N2 - Stimulation of purified human eosinophiis with 50 μM arachidonic acid leads to the production of leukotriene C4, 15-hydroxy-eicosatetraenoic acid and 15-series leukotrienes. The ratio of the amounts of leukotriene C4 and 15-lipoxygenase products was found to be strongly dependent on the arachidonic acid concentration, being relatively large at low arachidonic acid concentrations and very small at high arachidonic acid concentrations. In the presence of 1 μM platelet-activating factor a significant elevation of leukotriene C4 formation is observed, whereas the formation of 15-lipoxygenase products remains unaltered. As arachidonic acid was found to be capable of inducing a fast, transient rise in the cytosolic free Ca2+ concentration, this explains at least partly its ability to induce the Ca2+-dependent formation of leukotriene C4.
AB - Stimulation of purified human eosinophiis with 50 μM arachidonic acid leads to the production of leukotriene C4, 15-hydroxy-eicosatetraenoic acid and 15-series leukotrienes. The ratio of the amounts of leukotriene C4 and 15-lipoxygenase products was found to be strongly dependent on the arachidonic acid concentration, being relatively large at low arachidonic acid concentrations and very small at high arachidonic acid concentrations. In the presence of 1 μM platelet-activating factor a significant elevation of leukotriene C4 formation is observed, whereas the formation of 15-lipoxygenase products remains unaltered. As arachidonic acid was found to be capable of inducing a fast, transient rise in the cytosolic free Ca2+ concentration, this explains at least partly its ability to induce the Ca2+-dependent formation of leukotriene C4.
KW - fMLP
KW - H(P)ETE
KW - hydro(pero)xy-eicosatetraenoic acid
KW - leukotriene C
KW - LTC
KW - N-formyl-methionyl-leucyl-phenylalanine
KW - NDGA
KW - nordihydroguaiaretic acid
KW - PAF
KW - platelet-activatingfactor
KW - reversed-phase high performance liquid chromatography
KW - RP-HPLC
UR - https://www.scopus.com/pages/publications/0023943081
U2 - 10.1016/S0006-291X(88)81148-3
DO - 10.1016/S0006-291X(88)81148-3
M3 - Article
C2 - 3132917
AN - SCOPUS:0023943081
SN - 0006-291X
VL - 153
SP - 676
EP - 682
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -