TY - JOUR
T1 - Apoptosis, toll-like, RIG-I-like and NOD-like receptors are pathways jointly induced by diverse respiratory bacterial and viral pathogens
AU - Martínez, Isidoro
AU - Oliveros, Juan C.
AU - Cuesta, Isabel
AU - de la Barrera, Jorge
AU - Ausina, Vicente
AU - Casals, Cristina
AU - de Lorenzo, Alba
AU - García, Ernesto
AU - García-Fojeda, Belén
AU - Garmendia, Junkal
AU - González-Nicolau, Mar
AU - Lacoma, Alicia
AU - Menéndez, Margarita
AU - Moranta, David
AU - Nieto, Amelia
AU - Ortín, Juan
AU - Pérez-González, Alicia
AU - Prat, Cristina
AU - Ramos-Sevillano, Elisa
AU - Regueiro, Verónica
AU - Rodriguez-Frandsen, Ariel
AU - Solís, Dolores
AU - Yuste, José
AU - Bengoechea, José A.
AU - Melero, José A.
N1 - Funding Information:
We gratefully acknowledge financial support from the "CIBER de Enfermedades Respiratorias" (CIBERES), an initiative of the "Instituto de Salud Carlos III" (ISCIII), Spain. Research activities in the participating laboratories received further funding from the following sources: Centro Nacional de Microbiología, ISCIII, PI15CIII/00024 and MINECO (SAF2015-67033-R); Centro Nacional de Biotecnología, MINECO (BFU2014-57797-R); Hospital Universitari Germans Trias I Pujol, Spanish Society of Pneumology and Thoracic Surgery (SEPAR 054/2011); Departamento de Bioquímica y Biología Molecular I, MINECO (SAF2015-65307-R); Centro de Investigaciones Biológicas, MINECO (SAF2012-39444-C01/02); Fundación de Investigación Sanitaria de las Islas Baleares, MINECO (SAF2012-39841); Instituto de Agrobiotecnología, MINECO (SAF2015-66520-R); Instituto de Química Física Rocasolano, MINECO (BFU2015-70052-R) and the Marie Curie Initial Training Network GLYCOPHARM (PITN-GA-2012-317297). Subprograma Estatal de Formación (BES-2013-065355).
Publisher Copyright:
© 2017 Martínez, Oliveros, Cuesta, de la Barrera, Ausina, Casals, de Lorenzo, García, García-Fojeda, Garmendia, González-Nicolau, Lacoma, Menéndez, Moranta, Nieto, Ortín, Pérez-González, Prat, Ramos-Sevillano, Regueiro, Rodriguez-Frandsen, Solís, Yuste, Bengoechea and Melero.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Lower respiratory tract infections are among the top five leading causes of human death. Fighting these infections is therefore a world health priority. Searching for induced alterations in host gene expression shared by several relevant respiratory pathogens represents an alternative to identify new targets for wide-range host-oriented therapeutics. With this aim, alveolar macrophages were independently infected with three unrelated bacterial (Streptococcus pneumoniae, Klebsiella pneumoniae, and Staphylococcus aureus) and two dissimilar viral (respiratory syncytial virus and influenza A virus) respiratory pathogens, all of them highly relevant for human health. Cells were also activated with bacterial lipopolysaccharide (LPS) as a prototypical pathogen-associated molecular pattern. Patterns of differentially expressed cellular genes shared by the indicated pathogens were searched by microarray analysis. Most of the commonly up-regulated host genes were related to the innate immune response and/or apoptosis, with Toll-like, RIG-I-like and NOD-like receptors among the top 10 signaling pathways with over-expressed genes. These results identify new potential broad-spectrum targets to fight the important human infections caused by the bacteria and viruses studied here.
AB - Lower respiratory tract infections are among the top five leading causes of human death. Fighting these infections is therefore a world health priority. Searching for induced alterations in host gene expression shared by several relevant respiratory pathogens represents an alternative to identify new targets for wide-range host-oriented therapeutics. With this aim, alveolar macrophages were independently infected with three unrelated bacterial (Streptococcus pneumoniae, Klebsiella pneumoniae, and Staphylococcus aureus) and two dissimilar viral (respiratory syncytial virus and influenza A virus) respiratory pathogens, all of them highly relevant for human health. Cells were also activated with bacterial lipopolysaccharide (LPS) as a prototypical pathogen-associated molecular pattern. Patterns of differentially expressed cellular genes shared by the indicated pathogens were searched by microarray analysis. Most of the commonly up-regulated host genes were related to the innate immune response and/or apoptosis, with Toll-like, RIG-I-like and NOD-like receptors among the top 10 signaling pathways with over-expressed genes. These results identify new potential broad-spectrum targets to fight the important human infections caused by the bacteria and viruses studied here.
KW - Bacterial infections
KW - Core of up-regulated genes
KW - Host response
KW - Respiratory pathogens
KW - Viral infections
UR - https://www.scopus.com/pages/publications/85018302835
U2 - 10.3389/fmicb.2017.00276
DO - 10.3389/fmicb.2017.00276
M3 - Article
AN - SCOPUS:85018302835
SN - 1664-302X
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 276
ER -