Antibody-coated target cell membrane-induced chemiluminescence by human polymorphonuclear leukocytes

Kok P.M. Van Kessel; Jos A.G. Van Strijp; Lia M. Miltenburg; Henny J. Van Kats-Renaud; Ad C. Fluit; Jan Verhoef

doi:10.1016/0022-1759(89)90017-3

Antibody-coated target cell membrane-induced chemiluminescence by human polymorphonuclear leukocytes

Kok P.M. Van Kessel^*, Jos A.G. Van Strijp, Lia M. Miltenburg, Henny J. Van Kats-Renaud, Ad C. Fluit, Jan Verhoef

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

4 Citations (Scopus)

Abstract

Activation of the oxidative metabolic burst of human polymorphonuclear leukocytes (PMN) by antibody-coated crude membrane fragments of K562 tumor cells was measured in a luminometer. Induction of the chemiluminescence (Cl) response was measured in the presence of luminol and lucigenin. The Cl was dependent on the concentration of PMN, the enhancer luminol or lucigenin, and the amount of tumor cell fragments and anti-K562 serum. PMN were not triggered to a Cl response in the absence of antibodies. The lucigenin-enhanced Cl involved superoxide anion detection while the luminol-enhanced Cl was dependent on the presence of myeloperoxidase and involved hydroxyl radicals. An intact cytoskeleton and energy were necessary for an optima Cl response.

Original language	English
Pages (from-to)	279-285
Number of pages	7
Journal	Journal of Immunological Methods
Volume	118
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(89)90017-3
Publication status	Published - 31 Mar 1989

Keywords

Antibody-dependent cell-mediated cytotoxicity
Chemiluminescence
Fc receptor
Polymorphonuclear leukocyte
Target cell fragment

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10.1016/0022-1759(89)90017-3

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title = "Antibody-coated target cell membrane-induced chemiluminescence by human polymorphonuclear leukocytes",

abstract = "Activation of the oxidative metabolic burst of human polymorphonuclear leukocytes (PMN) by antibody-coated crude membrane fragments of K562 tumor cells was measured in a luminometer. Induction of the chemiluminescence (Cl) response was measured in the presence of luminol and lucigenin. The Cl was dependent on the concentration of PMN, the enhancer luminol or lucigenin, and the amount of tumor cell fragments and anti-K562 serum. PMN were not triggered to a Cl response in the absence of antibodies. The lucigenin-enhanced Cl involved superoxide anion detection while the luminol-enhanced Cl was dependent on the presence of myeloperoxidase and involved hydroxyl radicals. An intact cytoskeleton and energy were necessary for an optima Cl response.",

keywords = "Antibody-dependent cell-mediated cytotoxicity, Chemiluminescence, Fc receptor, Polymorphonuclear leukocyte, Target cell fragment",

author = "{Van Kessel}, {Kok P.M.} and {Van Strijp}, {Jos A.G.} and Miltenburg, {Lia M.} and {Van Kats-Renaud}, {Henny J.} and Fluit, {Ad C.} and Jan Verhoef",

year = "1989",

month = mar,

day = "31",

doi = "10.1016/0022-1759(89)90017-3",

language = "English",

volume = "118",

pages = "279--285",

journal = "Journal of Immunological Methods",

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TY - JOUR

T1 - Antibody-coated target cell membrane-induced chemiluminescence by human polymorphonuclear leukocytes

AU - Van Kessel, Kok P.M.

AU - Van Strijp, Jos A.G.

AU - Miltenburg, Lia M.

AU - Van Kats-Renaud, Henny J.

AU - Fluit, Ad C.

AU - Verhoef, Jan

PY - 1989/3/31

Y1 - 1989/3/31

N2 - Activation of the oxidative metabolic burst of human polymorphonuclear leukocytes (PMN) by antibody-coated crude membrane fragments of K562 tumor cells was measured in a luminometer. Induction of the chemiluminescence (Cl) response was measured in the presence of luminol and lucigenin. The Cl was dependent on the concentration of PMN, the enhancer luminol or lucigenin, and the amount of tumor cell fragments and anti-K562 serum. PMN were not triggered to a Cl response in the absence of antibodies. The lucigenin-enhanced Cl involved superoxide anion detection while the luminol-enhanced Cl was dependent on the presence of myeloperoxidase and involved hydroxyl radicals. An intact cytoskeleton and energy were necessary for an optima Cl response.

AB - Activation of the oxidative metabolic burst of human polymorphonuclear leukocytes (PMN) by antibody-coated crude membrane fragments of K562 tumor cells was measured in a luminometer. Induction of the chemiluminescence (Cl) response was measured in the presence of luminol and lucigenin. The Cl was dependent on the concentration of PMN, the enhancer luminol or lucigenin, and the amount of tumor cell fragments and anti-K562 serum. PMN were not triggered to a Cl response in the absence of antibodies. The lucigenin-enhanced Cl involved superoxide anion detection while the luminol-enhanced Cl was dependent on the presence of myeloperoxidase and involved hydroxyl radicals. An intact cytoskeleton and energy were necessary for an optima Cl response.

KW - Antibody-dependent cell-mediated cytotoxicity

KW - Chemiluminescence

KW - Fc receptor

KW - Polymorphonuclear leukocyte

KW - Target cell fragment

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U2 - 10.1016/0022-1759(89)90017-3

DO - 10.1016/0022-1759(89)90017-3

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C2 - 2926157

AN - SCOPUS:0024594639

SN - 0022-1759

VL - 118

SP - 279

EP - 285

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

Antibody-coated target cell membrane-induced chemiluminescence by human polymorphonuclear leukocytes

Abstract

Keywords

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