Antibodies enhance CXCL10 production during RSV infection of infant and adult immune cells

Marloes Vissers; Inge Schreurs; Jop Jans; Jacco Heldens; Ronald de Groot; Marien I de Jonge; Gerben Ferwerda

doi:10.1016/j.cyto.2015.07.024

Antibodies enhance CXCL10 production during RSV infection of infant and adult immune cells

Marloes Vissers, Inge Schreurs, Jop Jans, Jacco Heldens, Ronald de Groot, Marien I de Jonge, Gerben Ferwerda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Respiratory syncytial virus (RSV) bronchiolitis is a major burden in infants below three months of age, when the primary immune response is mainly dependent on innate immunity and maternal antibodies. We investigated the influence of antibodies on innate immunity during RSV infection. PBMCs from infants and adults were stimulated with live RSV and inactivated RSV in combination with antibody-containing and antibody-depleted serum. The immune response was determined by transcriptome analysis and chemokine levels were measured using ELISA and flow cytometry. Microarray data showed that CXCL10 gene transcription was RSV dependent, whereas CXCL11 and IFNα were upregulated in an antibody-dependent manner. Although the presence of antibodies reduces RSV infection rate, it enhances the innate immune response. In adult immune cells, antibodies enhance CXCL10, CXCL11, IFNα and IFNγ production in response to RSV infection. Contrary, in infant immune cells only CXCL10 was enhanced in an antibody-dependent manner. Monocytes are the main source of CXCL10 and they produce CXCL10 in both an antibody- and virus-dependent manner. This study shows that antibodies enhance CXCL10 production in infant immune cells. CXCL10 has been implicated in exuberating the inflammatory response during viral infections and antibodies could therefore play a role in the pathogenesis of RSV infections.

Original language	English
Pages (from-to)	458-464
Number of pages	7
Journal	Cytokine
Volume	76
Issue number	2
DOIs	https://doi.org/10.1016/j.cyto.2015.07.024
Publication status	Published - Dec 2015
Externally published	Yes

Keywords

Adult
Antibodies/immunology
Chemokine CXCL10/genetics
Cytokines/biosynthesis
Enzyme-Linked Immunosorbent Assay
Humans
Immunity, Innate
Infant
Respiratory Syncytial Virus Infections/immunology
Transcription, Genetic

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10.1016/j.cyto.2015.07.024

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title = "Antibodies enhance CXCL10 production during RSV infection of infant and adult immune cells",

abstract = "Respiratory syncytial virus (RSV) bronchiolitis is a major burden in infants below three months of age, when the primary immune response is mainly dependent on innate immunity and maternal antibodies. We investigated the influence of antibodies on innate immunity during RSV infection. PBMCs from infants and adults were stimulated with live RSV and inactivated RSV in combination with antibody-containing and antibody-depleted serum. The immune response was determined by transcriptome analysis and chemokine levels were measured using ELISA and flow cytometry. Microarray data showed that CXCL10 gene transcription was RSV dependent, whereas CXCL11 and IFNα were upregulated in an antibody-dependent manner. Although the presence of antibodies reduces RSV infection rate, it enhances the innate immune response. In adult immune cells, antibodies enhance CXCL10, CXCL11, IFNα and IFNγ production in response to RSV infection. Contrary, in infant immune cells only CXCL10 was enhanced in an antibody-dependent manner. Monocytes are the main source of CXCL10 and they produce CXCL10 in both an antibody- and virus-dependent manner. This study shows that antibodies enhance CXCL10 production in infant immune cells. CXCL10 has been implicated in exuberating the inflammatory response during viral infections and antibodies could therefore play a role in the pathogenesis of RSV infections.",

keywords = "Adult, Antibodies/immunology, Chemokine CXCL10/genetics, Cytokines/biosynthesis, Enzyme-Linked Immunosorbent Assay, Humans, Immunity, Innate, Infant, Respiratory Syncytial Virus Infections/immunology, Transcription, Genetic",

author = "Marloes Vissers and Inge Schreurs and Jop Jans and Jacco Heldens and {de Groot}, Ronald and {de Jonge}, {Marien I} and Gerben Ferwerda",

year = "2015",

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doi = "10.1016/j.cyto.2015.07.024",

language = "English",

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pages = "458--464",

journal = "Cytokine",

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T1 - Antibodies enhance CXCL10 production during RSV infection of infant and adult immune cells

AU - Vissers, Marloes

AU - Schreurs, Inge

AU - Jans, Jop

AU - Heldens, Jacco

AU - de Groot, Ronald

AU - de Jonge, Marien I

AU - Ferwerda, Gerben

PY - 2015/12

Y1 - 2015/12

N2 - Respiratory syncytial virus (RSV) bronchiolitis is a major burden in infants below three months of age, when the primary immune response is mainly dependent on innate immunity and maternal antibodies. We investigated the influence of antibodies on innate immunity during RSV infection. PBMCs from infants and adults were stimulated with live RSV and inactivated RSV in combination with antibody-containing and antibody-depleted serum. The immune response was determined by transcriptome analysis and chemokine levels were measured using ELISA and flow cytometry. Microarray data showed that CXCL10 gene transcription was RSV dependent, whereas CXCL11 and IFNα were upregulated in an antibody-dependent manner. Although the presence of antibodies reduces RSV infection rate, it enhances the innate immune response. In adult immune cells, antibodies enhance CXCL10, CXCL11, IFNα and IFNγ production in response to RSV infection. Contrary, in infant immune cells only CXCL10 was enhanced in an antibody-dependent manner. Monocytes are the main source of CXCL10 and they produce CXCL10 in both an antibody- and virus-dependent manner. This study shows that antibodies enhance CXCL10 production in infant immune cells. CXCL10 has been implicated in exuberating the inflammatory response during viral infections and antibodies could therefore play a role in the pathogenesis of RSV infections.

AB - Respiratory syncytial virus (RSV) bronchiolitis is a major burden in infants below three months of age, when the primary immune response is mainly dependent on innate immunity and maternal antibodies. We investigated the influence of antibodies on innate immunity during RSV infection. PBMCs from infants and adults were stimulated with live RSV and inactivated RSV in combination with antibody-containing and antibody-depleted serum. The immune response was determined by transcriptome analysis and chemokine levels were measured using ELISA and flow cytometry. Microarray data showed that CXCL10 gene transcription was RSV dependent, whereas CXCL11 and IFNα were upregulated in an antibody-dependent manner. Although the presence of antibodies reduces RSV infection rate, it enhances the innate immune response. In adult immune cells, antibodies enhance CXCL10, CXCL11, IFNα and IFNγ production in response to RSV infection. Contrary, in infant immune cells only CXCL10 was enhanced in an antibody-dependent manner. Monocytes are the main source of CXCL10 and they produce CXCL10 in both an antibody- and virus-dependent manner. This study shows that antibodies enhance CXCL10 production in infant immune cells. CXCL10 has been implicated in exuberating the inflammatory response during viral infections and antibodies could therefore play a role in the pathogenesis of RSV infections.

KW - Adult

KW - Antibodies/immunology

KW - Chemokine CXCL10/genetics

KW - Cytokines/biosynthesis

KW - Enzyme-Linked Immunosorbent Assay

KW - Humans

KW - Immunity, Innate

KW - Infant

KW - Respiratory Syncytial Virus Infections/immunology

KW - Transcription, Genetic

U2 - 10.1016/j.cyto.2015.07.024

DO - 10.1016/j.cyto.2015.07.024

M3 - Article

C2 - 26253531

SN - 1043-4666

VL - 76

SP - 458

EP - 464

JO - Cytokine

JF - Cytokine

IS - 2

ER -

Antibodies enhance CXCL10 production during RSV infection of infant and adult immune cells

Abstract

Keywords

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