Antibodies and complement enhance binding and uptake of HIV-1 by human monocytes

L. J. Bakker*, H. S L M Nottet, N. M. De Vos, L. De Graaf, J. A G Van Strijp, M. R. Visser, J. Verhoef

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Objective: To characterize antibody- and complement-mediated binding and uptake of HIV-1 by human monocytes. Design: The first step in the infection of the monocyte by HIV-1 is binding of the virus to the susceptible cell. Procedures were designed to assess the influence of anti-HIV-1 antibodies and complement on this binding, and to study the process of internalization following binding. Methods: Human monocytes were incubated with fluorescein-labelled purified HTLV-III(B) virions and human sera with high-titre anti-HIV-1 antibodies and/or complement. Binding and uptake of virus by the monocytes was measured as fluorescence per cell by flow cytometry. Results: Binding of purified HIV-1 to monocytes was increased by complement and, to a lesser extent, by anti-HIV-1 antibodies. Uptake of HIV-1 bound to the monocyte appeared to be mediated by antibodies and was increased further by the presence of complement. Complement alone, however, resulted in the uptake of only a small part of the bound virus. Conclusions: Complement significantly increases the binding of HIV-1 to human monocytes, and a combination of antibodies and complement efficiently mediates uptake of HIV-1 by monocytes.

Original languageEnglish
Pages (from-to)35-41
Number of pages7
JournalAIDS
Volume6
Issue number1
Publication statusPublished - 1 Jan 1992

Keywords

  • Antibody
  • Complement
  • Flow cytometry
  • HIV-1 binding
  • Monocyte
  • Neutrophil
  • Opsonization
  • Phagocytosis

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