@article{4ec2d5a228774618a9dfe46c19dceadc,
title = "Antibacterial and anti-inflammatory properties of host defense peptides against Staphylococcus aureus",
abstract = "Cationic host defense peptides (HDPs) are a promising alternative to antibiotics in the fight against Staphylococcus aureus infections. In this study, we investigated the antibacterial and immunomodulatory properties of three HDPs namely IDR-1018, CATH-2, and LL-37. Although all three HDPs significantly inhibited LPS-induced activation of human macrophages, only CATH-2 prevented S. aureus growth. When applied to different infection models focused on intracellularly surviving bacteria, only IDR-1018 showed a consistent reduction in macrophage bacterial uptake. However, this observation did not correlate with an increase in killing the efficiency of intracellular S. aureus. Here, we conclude that despite the promising antibacterial and anti-inflammatory properties of the selected HDPs, macrophages{\textquoteright} intrinsic antibacterial functions were not improved. Future studies should either focus on combining different HDPs or using them synergistically with other antibacterial agents to improve immune cells{\textquoteright} efficacy against S. aureus pathogenesis.",
keywords = "Immunology, Microbiology",
author = "Leonardo Cecotto and {van Kessel}, Kok and Wolfert, {Margreet A.} and Charles Vogely and {van der Wal}, Bart and Harrie Weinans and {van Strijp}, Jos and {Amin Yavari}, Saber",
note = "Funding Information: We thank Lisanne de Vor for assistance with acquiring images by confocal microscopy, and Dr. Michiel Croes for the meaningful discussions and data interpretations during the first stages of the research. The collaboration project is cofounded by the PPP Allowance made available by Health ∼ Holland, Top Sector Life Sciences & Health, to stimulate public-private partnerships. This research also received co-funding from the EU's H2020 research and innovation program under Marie S. Curie co-fund RESCUE grant agreement No 801540. This publication is part of the project DARTBAC (with project number NWA.1292.19.354 of the research program NWA-ORC which is (partly) financed by the Dutch Research Council (NWO). Conceptualization, S.A.; Methodology L.C. K.K. J.S. H.W. and S.A.; Investigation L.C. and M.W.; Writing – Original draft, L.C. K.K. J.S. and S.A; Writing – Review & Editing, L.C. K.K. M.W, C.V. B.W. H.W. J.S. and S.A; Funding Acquisition, H.W. and S.A.; Resources, K.K. H.W. J.S. and S.A; Supervision, K.K. H.W. J.S. and S.A. The authors declare no competing interests. Funding Information: We thank Lisanne de Vor for assistance with acquiring images by confocal microscopy, and Dr. Michiel Croes for the meaningful discussions and data interpretations during the first stages of the research. The collaboration project is cofounded by the PPP Allowance made available by Health ∼ Holland, Top Sector Life Sciences & Health, to stimulate public-private partnerships. This research also received co-funding from the EU{\textquoteright}s H2020 research and innovation program under Marie S. Curie co-fund RESCUE grant agreement No 801540 . This publication is part of the project DARTBAC (with project number NWA.1292.19.354 of the research program NWA-ORC which is (partly) financed by the Dutch Research Council (NWO). Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = oct,
day = "21",
doi = "10.1016/j.isci.2022.105211",
language = "English",
volume = "25",
pages = "1--17",
number = "10",
}