Analysis of buoyant density of canine peripheral blood leukocytes with PVP-Silica (Percoll) density gradients

W. A. Buurman; P. A. Vegt; G. Groenewegen; C. J v d Linden; G. M A A Jeunhomme

doi:10.1016/0165-2427(82)90038-1

Analysis of buoyant density of canine peripheral blood leukocytes with PVP-Silica (Percoll) density gradients

W. A. Buurman^*, P. A. Vegt, G. Groenewegen, C. J v d Linden, G. M A A Jeunhomme

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

Isolation of canine peripheral blood mononuclear cells with a one step centrifugal separation procedure has not been very succesful sofar. Significant contamination with polymorphonuclear cells has been reported. An analysis of the buoyant density of canine peripheral blood leukocytes on a self-generating Percoll gradient showed that the buoyant densities of polymorphonuclear cells and lymphocytes are so near that separation with high purity and yield is not possible with the use of a density gradient. Transient changes in buoyant density of polymorphonuclear cells have been observed. In such situations differences in buoyant density between cell types have been observed which permit separation of mononuclear cells from polymorphonuclear cells at a reasonable yield.

Original language	English
Pages (from-to)	547-556
Number of pages	10
Journal	Veterinary Immunology and Immunopathology
Volume	3
Issue number	6
DOIs	https://doi.org/10.1016/0165-2427(82)90038-1
Publication status	Published - 1 Jan 1982

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title = "Analysis of buoyant density of canine peripheral blood leukocytes with PVP-Silica (Percoll) density gradients",

abstract = "Isolation of canine peripheral blood mononuclear cells with a one step centrifugal separation procedure has not been very succesful sofar. Significant contamination with polymorphonuclear cells has been reported. An analysis of the buoyant density of canine peripheral blood leukocytes on a self-generating Percoll gradient showed that the buoyant densities of polymorphonuclear cells and lymphocytes are so near that separation with high purity and yield is not possible with the use of a density gradient. Transient changes in buoyant density of polymorphonuclear cells have been observed. In such situations differences in buoyant density between cell types have been observed which permit separation of mononuclear cells from polymorphonuclear cells at a reasonable yield.",

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AU - Vegt, P. A.

AU - Groenewegen, G.

AU - Linden, C. J v d

AU - Jeunhomme, G. M A A

PY - 1982/1/1

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N2 - Isolation of canine peripheral blood mononuclear cells with a one step centrifugal separation procedure has not been very succesful sofar. Significant contamination with polymorphonuclear cells has been reported. An analysis of the buoyant density of canine peripheral blood leukocytes on a self-generating Percoll gradient showed that the buoyant densities of polymorphonuclear cells and lymphocytes are so near that separation with high purity and yield is not possible with the use of a density gradient. Transient changes in buoyant density of polymorphonuclear cells have been observed. In such situations differences in buoyant density between cell types have been observed which permit separation of mononuclear cells from polymorphonuclear cells at a reasonable yield.

AB - Isolation of canine peripheral blood mononuclear cells with a one step centrifugal separation procedure has not been very succesful sofar. Significant contamination with polymorphonuclear cells has been reported. An analysis of the buoyant density of canine peripheral blood leukocytes on a self-generating Percoll gradient showed that the buoyant densities of polymorphonuclear cells and lymphocytes are so near that separation with high purity and yield is not possible with the use of a density gradient. Transient changes in buoyant density of polymorphonuclear cells have been observed. In such situations differences in buoyant density between cell types have been observed which permit separation of mononuclear cells from polymorphonuclear cells at a reasonable yield.

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JO - Veterinary Immunology and Immunopathology

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ER -

Analysis of buoyant density of canine peripheral blood leukocytes with PVP-Silica (Percoll) density gradients

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