An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic golgi fragments

Catherine Rabouille; Timothy P. Levine; Jan Michael Peters; Graham Warren

doi:10.1016/0092-8674(95)90270-8

An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic golgi fragments

Catherine Rabouille^*
, Timothy P. Levine
, Jan Michael Peters
, Graham Warren

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

297 Citations (Scopus)

Abstract

Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.

Original language	English
Pages (from-to)	905-914
Number of pages	10
Journal	Cell
Volume	82
Issue number	6
DOIs	https://doi.org/10.1016/0092-8674(95)90270-8
Publication status	Published - 22 Sept 1995

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title = "An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic golgi fragments",

abstract = "Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.",

author = "Catherine Rabouille and Levine, \{Timothy P.\} and Peters, \{Jan Michael\} and Graham Warren",

year = "1995",

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AU - Rabouille, Catherine

AU - Levine, Timothy P.

AU - Peters, Jan Michael

AU - Warren, Graham

PY - 1995/9/22

Y1 - 1995/9/22

N2 - Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.

AB - Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.

UR - https://www.scopus.com/pages/publications/0029089959

U2 - 10.1016/0092-8674(95)90270-8

DO - 10.1016/0092-8674(95)90270-8

M3 - Article

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AN - SCOPUS:0029089959

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SP - 905

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JO - Cell

JF - Cell

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ER -

An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic golgi fragments

Abstract

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