TY - JOUR
T1 - An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic golgi fragments
AU - Rabouille, Catherine
AU - Levine, Timothy P.
AU - Peters, Jan Michael
AU - Warren, Graham
PY - 1995/9/22
Y1 - 1995/9/22
N2 - Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.
AB - Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.
UR - http://www.scopus.com/inward/record.url?scp=0029089959&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(95)90270-8
DO - 10.1016/0092-8674(95)90270-8
M3 - Article
C2 - 7553851
AN - SCOPUS:0029089959
SN - 0092-8674
VL - 82
SP - 905
EP - 914
JO - Cell
JF - Cell
IS - 6
ER -