Abstract
A new method for the separation of murine peritoneal macrophage populations is described. Different macrophage populations were separated by velocity sedimentation in a Percoll gradient. The macrophages were separated by size as was shown by a morphometrical analysis. The different macrophage populations differed in their enzyme cytochemical profile. The overall recovery of peritoneal cells is more than 95% and the macrophages adhered normally to glass surfaces after washing. This indicated that this l X g sedimentation separation of macrophages, which is relatively simple to perform, is suitable for the study of macrophage heterogeneity.
| Original language | English |
|---|---|
| Pages (from-to) | 117-23 |
| Number of pages | 7 |
| Journal | Journal of Immunological Methods |
| Volume | 61 |
| Issue number | 1 |
| Publication status | Published - 24 Jun 1983 |
Keywords
- Animals
- Cell Nucleus
- Cell Separation
- Centrifugation, Density Gradient
- Female
- Macrophages
- Mice
- Mice, Inbred Strains