Abstract
The mRNAs encoding the Rev and Env proteins of simian immunodeficiency virus (SIV) are unique because upstream translation start codons are present that may modulate the expression of these viral proteins. We previously reported the regulatory effect of a small upstream open reading frame (ORF) on Rev and Env translation. Here we study this mechanism in further detail by modulating the strength of the translation signals upstream of the open reading frames in subgenomic reporters. Furthermore, the effects of these mutations on SIV gene expression and viral replication are analyzed. An intricate regulatory mechanism is disclosed that allows the virus to express a balanced amount of these two proteins.
Original language | English |
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Pages (from-to) | 191-200 |
Number of pages | 10 |
Journal | Virology |
Volume | 436 |
Issue number | 1 |
DOIs | |
Publication status | Published - 5 Feb 2013 |
Externally published | Yes |
Keywords
- Cell Line
- Codon, Initiator
- Gene Expression Regulation, Viral
- Gene Products, env/genetics
- Gene Products, rev/genetics
- Genes, env
- Genes, rev
- HEK293 Cells
- Humans
- Mutation
- Open Reading Frames
- Protein Biosynthesis
- RNA, Messenger/genetics
- Simian Immunodeficiency Virus/genetics