An AP-1 site in the promoter of the human IL-5Rα gene is necessary for promoter activity in eosinophilic HL60 cells

Belinda Baltus; Thamar B. Van Dijk; Eric Caldenhoven; Ed Zanders; J. A M Raaijmakers; J. J. Lammers; Leo Koenderman; Rolf P. De Groot

doi:10.1016/S0014-5793(98)00991-0

An AP-1 site in the promoter of the human IL-5Rα gene is necessary for promoter activity in eosinophilic HL60 cells

Belinda Baltus, Thamar B. Van Dijk, Eric Caldenhoven, Ed Zanders, J. A M Raaijmakers, J. J. Lammers, Leo Koenderman, Rolf P. De Groot^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

11 Citations (Scopus)

Abstract

Interleukin-5 (IL-5) plays a crucial role in the proliferation, differentiation and activation of eosinophils. The IL-5 receptor is composed of an IL-5-specific α subunit, which is expressed by eosinophils and basophils, and a βc-subunit shared with the receptors for IL-3 and GM-CSF. We identified an AP-1 element which is important for IL-5Rα promoter activity in eosinophilic HL60 cells. The AP-1 site and the previously identified EOS1 site cooperate, since single mutation of either of the sites decreased promoter activity. We show that the AP-1 site of the IL-5Rα promoter binds multiple proteins, including cJun, CREB, and CREM. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	251-254
Number of pages	4
Journal	FEBS letters
Volume	434
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00991-0
Publication status	Published - 4 Sept 1998

Keywords

AP-1
Eosinophil
Interleukin-5 receptor
Transcription regulation

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10.1016/S0014-5793(98)00991-0

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title = "An AP-1 site in the promoter of the human IL-5Rα gene is necessary for promoter activity in eosinophilic HL60 cells",

abstract = "Interleukin-5 (IL-5) plays a crucial role in the proliferation, differentiation and activation of eosinophils. The IL-5 receptor is composed of an IL-5-specific α subunit, which is expressed by eosinophils and basophils, and a βc-subunit shared with the receptors for IL-3 and GM-CSF. We identified an AP-1 element which is important for IL-5Rα promoter activity in eosinophilic HL60 cells. The AP-1 site and the previously identified EOS1 site cooperate, since single mutation of either of the sites decreased promoter activity. We show that the AP-1 site of the IL-5Rα promoter binds multiple proteins, including cJun, CREB, and CREM. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "AP-1, Eosinophil, Interleukin-5 receptor, Transcription regulation",

author = "Belinda Baltus and {Van Dijk}, {Thamar B.} and Eric Caldenhoven and Ed Zanders and Raaijmakers, {J. A M} and Lammers, {J. J.} and Leo Koenderman and {De Groot}, {Rolf P.}",

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T1 - An AP-1 site in the promoter of the human IL-5Rα gene is necessary for promoter activity in eosinophilic HL60 cells

AU - Baltus, Belinda

AU - Van Dijk, Thamar B.

AU - Caldenhoven, Eric

AU - Zanders, Ed

AU - Raaijmakers, J. A M

AU - Lammers, J. J.

AU - Koenderman, Leo

AU - De Groot, Rolf P.

PY - 1998/9/4

Y1 - 1998/9/4

N2 - Interleukin-5 (IL-5) plays a crucial role in the proliferation, differentiation and activation of eosinophils. The IL-5 receptor is composed of an IL-5-specific α subunit, which is expressed by eosinophils and basophils, and a βc-subunit shared with the receptors for IL-3 and GM-CSF. We identified an AP-1 element which is important for IL-5Rα promoter activity in eosinophilic HL60 cells. The AP-1 site and the previously identified EOS1 site cooperate, since single mutation of either of the sites decreased promoter activity. We show that the AP-1 site of the IL-5Rα promoter binds multiple proteins, including cJun, CREB, and CREM. Copyright (C) 1998 Federation of European Biochemical Societies.

AB - Interleukin-5 (IL-5) plays a crucial role in the proliferation, differentiation and activation of eosinophils. The IL-5 receptor is composed of an IL-5-specific α subunit, which is expressed by eosinophils and basophils, and a βc-subunit shared with the receptors for IL-3 and GM-CSF. We identified an AP-1 element which is important for IL-5Rα promoter activity in eosinophilic HL60 cells. The AP-1 site and the previously identified EOS1 site cooperate, since single mutation of either of the sites decreased promoter activity. We show that the AP-1 site of the IL-5Rα promoter binds multiple proteins, including cJun, CREB, and CREM. Copyright (C) 1998 Federation of European Biochemical Societies.

KW - AP-1

KW - Eosinophil

KW - Interleukin-5 receptor

KW - Transcription regulation

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U2 - 10.1016/S0014-5793(98)00991-0

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AN - SCOPUS:0032407272

SN - 0014-5793

VL - 434

SP - 251

EP - 254

JO - FEBS letters

JF - FEBS letters

IS - 3

ER -

An AP-1 site in the promoter of the human IL-5Rα gene is necessary for promoter activity in eosinophilic HL60 cells

Abstract

Keywords

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