TY - JOUR
T1 - An Animal Model of Cutaneous Cyst Development Enables the Identification of Three Quantitative Trait Loci, Including the Homologue of a Human Locus (TRICY1)
AU - Cosgarea, I
AU - Koelsch, B
AU - Fischer, C
AU - Griewank, K G
AU - van den Berg, L
AU - Kutritz, A
AU - Schadendorf, D
AU - Kindler-Röhrborn, A
N1 - Funding Information:
This study was funded by the Federal Ministry of Research and Technology (BMBF) grant number 01FP1062/63 . The authors thank Armin Daschke and Marcus Linnenboden, Central Animal Facility, University Hospital of Essen, University of Duisburg-Essen, Essen, Germany, for expert assistance with animal husbandry.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Using inbred BN and LE/Stm rats susceptible and resistant, respectively, to chemically induced cutaneous cyst development we were able to further unveil the genetic architecture of inherited multiple cyst formation. N-methyl-N-nitrosourea-treated (BN x LE) F2 intercross rats proved to develop differential numbers of cutaneous cysts, demonstrating epidermal, trichilemmal and verrucous keratinization types. Male rats developed significantly more cysts per animal than females. QTL interval mapping yielded three loci on rat chromosomes 1, 8 and 11 (Ccd1, Ccd2, Ccd3) linked to cutaneous cyst formation. Ccd2 proved to be homologous to the human TRICY1 region which could further be narrowed down by genome comparison in both species. It contains 11 genes with evidence of expression in human keratinocytes.
AB - Using inbred BN and LE/Stm rats susceptible and resistant, respectively, to chemically induced cutaneous cyst development we were able to further unveil the genetic architecture of inherited multiple cyst formation. N-methyl-N-nitrosourea-treated (BN x LE) F2 intercross rats proved to develop differential numbers of cutaneous cysts, demonstrating epidermal, trichilemmal and verrucous keratinization types. Male rats developed significantly more cysts per animal than females. QTL interval mapping yielded three loci on rat chromosomes 1, 8 and 11 (Ccd1, Ccd2, Ccd3) linked to cutaneous cyst formation. Ccd2 proved to be homologous to the human TRICY1 region which could further be narrowed down by genome comparison in both species. It contains 11 genes with evidence of expression in human keratinocytes.
UR - http://www.scopus.com/inward/record.url?scp=85067888975&partnerID=8YFLogxK
U2 - 10.1016/j.jid.2019.03.1154
DO - 10.1016/j.jid.2019.03.1154
M3 - Article
C2 - 31039361
SN - 0022-202X
VL - 139
SP - 2235-2238.e5
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 10
ER -