@inbook{d50eba8ac7f349c2b9f23491d14c546f,
title = "Amplicon-Based Targeted Next-Generation Sequencing of Formalin-Fixed, Paraffin-Embedded Tissue",
abstract = "Next-generation sequencing (NGS) is rapidly becoming the method of choice for mutation analysis in both research and diagnostics. The benefit of targeted NGS compared to whole-genome and whole-exome sequencing is that smaller amounts of input material can be used as well as qualitatively suboptimal tissue samples, like formalin-fixed, paraffin-embedded archival tissue.Here, we describe the protocol for targeted next-generation sequencing using the Ion Torrent PGM platform in combination with Ion Ampliseq NGS gene panels for formalin-fixed, paraffin-embedded tissues. Both the manual and the automated workflow are described as well as the bioinformatics for data analysis.",
keywords = "Ampliseq, Formalin-fixed paraffin-embedded, Mutation analysis, Next-generation sequencing, Targeted sequencing, Variant calling",
author = "Eric Strengman and Barendrecht-Smouter, {Francoise A.S.} and {de Voijs}, Carmen and {de Vree}, Paula and Nijman, {Isaac J.} and {de Leng}, {Wendy W.J.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-9004-7_1",
language = "English",
isbn = "978-1-4939-9002-3",
volume = "1908",
series = "Methods in Molecular Biology",
publisher = "Springer Science + Business Media",
pages = "1--17",
editor = "Murray, {Sarah S.}",
booktitle = "Tumor profiling",
address = "United States",
}