TY - JOUR
T1 - Altered fibrin network structure and fibrinolysis in intensive care unit patients with COVID-19, not entirely explaining the increased risk of thrombosis
AU - de Vries, Judith J
AU - Visser, Chantal
AU - Geers, Lotte
AU - Slotman, Johan A
AU - van Kleef, Nadine D
AU - Maas, Coen
AU - Bax, Hannelore I
AU - Miedema, Jelle R
AU - van Gorp, Eric C M
AU - Goeijenbier, Marco
AU - van den Akker, Johannes P C
AU - Endeman, Henrik
AU - Rijken, Dingeman C
AU - Kruip, Marieke J H A
AU - de Maat, Moniek P M
N1 - Funding Information:
This work was supported by the Netherlands Thrombosis Foundation (Grant/Award Number: 2020_A) and The Netherlands Organization for Health Research and Development (Grant/Award Number: 10430012010004).
Funding Information:
This work was supported by the Netherlands Thrombosis Foundation (Grant/Award Number: 2020_A) and The Netherlands Organization for Health Research and Development (Grant/Award Number: 10430012010004). The authors thank Debby Priem-Visser, Aarazo Barakzie, and the technicians of the hemostasis laboratory for their excellent technical assistance. Furthermore, the authors thank all contributors from the Dutch COVID & Thrombosis Coalition (Appendix S1) for providing the framework for the current study.
Publisher Copyright:
© 2022 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis.
PY - 2022/6
Y1 - 2022/6
N2 - BACKGROUND: Severe acute respiratory syndrome coronavirus 2 infection is associated with an increased incidence of thrombosis.OBJECTIVES: By studying the fibrin network structure of coronavirus disease 2019 (COVID-19) patients, we aimed to unravel pathophysiological mechanisms that contribute to this increased risk of thrombosis. This may contribute to optimal prevention and treatment of COVID-19 related thrombosis.PATIENTS/METHODS: In this case-control study, we collected plasma samples from intensive care unit (ICU) patients with COVID-19, with and without confirmed thrombosis, between April and December 2020. Additionally, we collected plasma from COVID-19 patients admitted to general wards without thrombosis, from ICU patients with pneumococcal infection, and from healthy controls. Fibrin fiber diameters and fibrin network density were quantified in plasma clots imaged with stimulated emission depletion microscopy and confocal microscopy. Finally, we determined the sensitivity to fibrinolysis.RESULTS: COVID-19 ICU patients (n = 37) and ICU patients with pneumococcal disease (n = 7) showed significantly higher fibrin densities and longer plasma clot lysis times than healthy controls (n = 7). No differences were observed between COVID-19 ICU patients with and without thrombosis, or ICU patients with pneumococcal infection. At a second time point, after diagnosis of thrombosis or at a similar time point in patients without thrombosis, we observed thicker fibers and longer lysis times in COVID-19 ICU patients with thrombosis (n = 19) than in COVID-19 ICU patients without thrombosis (n = 18).CONCLUSIONS: Our results suggest that severe COVID-19 is associated with a changed fibrin network structure and decreased susceptibility to fibrinolysis. Because these changes were not exclusive to COVID-19 patients, they may not explain the increased thrombosis risk.
AB - BACKGROUND: Severe acute respiratory syndrome coronavirus 2 infection is associated with an increased incidence of thrombosis.OBJECTIVES: By studying the fibrin network structure of coronavirus disease 2019 (COVID-19) patients, we aimed to unravel pathophysiological mechanisms that contribute to this increased risk of thrombosis. This may contribute to optimal prevention and treatment of COVID-19 related thrombosis.PATIENTS/METHODS: In this case-control study, we collected plasma samples from intensive care unit (ICU) patients with COVID-19, with and without confirmed thrombosis, between April and December 2020. Additionally, we collected plasma from COVID-19 patients admitted to general wards without thrombosis, from ICU patients with pneumococcal infection, and from healthy controls. Fibrin fiber diameters and fibrin network density were quantified in plasma clots imaged with stimulated emission depletion microscopy and confocal microscopy. Finally, we determined the sensitivity to fibrinolysis.RESULTS: COVID-19 ICU patients (n = 37) and ICU patients with pneumococcal disease (n = 7) showed significantly higher fibrin densities and longer plasma clot lysis times than healthy controls (n = 7). No differences were observed between COVID-19 ICU patients with and without thrombosis, or ICU patients with pneumococcal infection. At a second time point, after diagnosis of thrombosis or at a similar time point in patients without thrombosis, we observed thicker fibers and longer lysis times in COVID-19 ICU patients with thrombosis (n = 19) than in COVID-19 ICU patients without thrombosis (n = 18).CONCLUSIONS: Our results suggest that severe COVID-19 is associated with a changed fibrin network structure and decreased susceptibility to fibrinolysis. Because these changes were not exclusive to COVID-19 patients, they may not explain the increased thrombosis risk.
KW - COVID-19
KW - Case-Control Studies
KW - Fibrin
KW - Fibrin Clot Lysis Time
KW - Fibrinolysis/physiology
KW - Humans
KW - Intensive Care Units
KW - Pneumococcal Infections/complications
KW - Thrombosis
UR - https://www.scopus.com/pages/publications/85127377789
U2 - 10.1111/jth.15708
DO - 10.1111/jth.15708
M3 - Article
C2 - 35316570
SN - 1538-7933
VL - 20
SP - 1412
EP - 1420
JO - Journal of thrombosis and haemostasis : JTH
JF - Journal of thrombosis and haemostasis : JTH
IS - 6
ER -