Allele-specific detection of single mRNA molecules in situ.

C.H. Hansen; A. van Oudenaarden

Allele-specific detection of single mRNA molecules in situ.

Translated title of the contribution: Allele-specific detection of single mRNA molecules in situ.

C.H. Hansen
, A. van Oudenaarden

Molecular Cancer Research

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.

Translated title of the contribution	Allele-specific detection of single mRNA molecules in situ.
Original language	Undefined/Unknown
Pages (from-to)	869-71
Number of pages	3
Journal	Nature Methods
Volume	10
Issue number	9
Publication status	Published - 2013

Cite this

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title = "Allele-specific detection of single mRNA molecules in situ.",

abstract = "We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95\% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.",

author = "C.H. Hansen and \{van Oudenaarden\}, A.",

year = "2013",

language = "Undefined/Unknown",

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pages = "869--71",

journal = "Nature Methods",

issn = "1548-7091",

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number = "9",

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T1 - Allele-specific detection of single mRNA molecules in situ.

AU - Hansen, C.H.

AU - van Oudenaarden, A.

PY - 2013

Y1 - 2013

N2 - We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.

AB - We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.

M3 - Article

SN - 1548-7091

VL - 10

SP - 869

EP - 871

JO - Nature Methods

JF - Nature Methods

IS - 9

ER -

Allele-specific detection of single mRNA molecules in situ.

Abstract

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