Adherence of human neutrophils changes Ca2+ signaling during activation with opsonized particles

Leo Koenderman; Anton T.J. Tool; Berend HooyBrink; Dirk Roos; Carl A. Hansen; John R. Williamson; Arthur J. Verhoeven

doi:10.1016/0014-5793(90)81232-D

Adherence of human neutrophils changes Ca²⁺ signaling during activation with opsonized particles

Leo Koenderman, Anton T.J. Tool, Berend HooyBrink, Dirk Roos, Carl A. Hansen, John R. Williamson, Arthur J. Verhoeven^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Changes in the cytosolic free Ca²⁺ concentration ([Ca²⁺]_i) upon activation of human neutrophils by opsonized particles (serum-treated zymosan; STZ) were evaluated by three different methods: (i) measurement of total fluorescence changes in indo-1 loaded neutrophils activated in suspension; (ii) measurement of fluorescence changes in individual indo-1 loaded neutrophils in a flow cytometer and (iii) measurement of fluorescence changes in individual fura-2 loaded neutrophils adherent to serum-coated coverslips. Our study shows that the opsonized particle-induced change in [Ca²⁺]_i in neutrophils is altered during adherence of the cells to a serum-coated surface. These observations might be of importance for neutrophil function in vivo, since adherence is a prerequisite for diapedesis and chemotaxis.

Original language	English
Pages (from-to)	49-52
Number of pages	4
Journal	FEBS letters
Volume	270
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)81232-D
Publication status	Published - 17 Sept 1990

Keywords

Adherence
Ca signaling
Neutrophil

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10.1016/0014-5793(90)81232-D

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title = "Adherence of human neutrophils changes Ca2+ signaling during activation with opsonized particles",

abstract = "Changes in the cytosolic free Ca2+ concentration ([Ca2+]i) upon activation of human neutrophils by opsonized particles (serum-treated zymosan; STZ) were evaluated by three different methods: (i) measurement of total fluorescence changes in indo-1 loaded neutrophils activated in suspension; (ii) measurement of fluorescence changes in individual indo-1 loaded neutrophils in a flow cytometer and (iii) measurement of fluorescence changes in individual fura-2 loaded neutrophils adherent to serum-coated coverslips. Our study shows that the opsonized particle-induced change in [Ca2+]i in neutrophils is altered during adherence of the cells to a serum-coated surface. These observations might be of importance for neutrophil function in vivo, since adherence is a prerequisite for diapedesis and chemotaxis.",

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AU - Koenderman, Leo

AU - Tool, Anton T.J.

AU - HooyBrink, Berend

AU - Roos, Dirk

AU - Hansen, Carl A.

AU - Williamson, John R.

AU - Verhoeven, Arthur J.

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N2 - Changes in the cytosolic free Ca2+ concentration ([Ca2+]i) upon activation of human neutrophils by opsonized particles (serum-treated zymosan; STZ) were evaluated by three different methods: (i) measurement of total fluorescence changes in indo-1 loaded neutrophils activated in suspension; (ii) measurement of fluorescence changes in individual indo-1 loaded neutrophils in a flow cytometer and (iii) measurement of fluorescence changes in individual fura-2 loaded neutrophils adherent to serum-coated coverslips. Our study shows that the opsonized particle-induced change in [Ca2+]i in neutrophils is altered during adherence of the cells to a serum-coated surface. These observations might be of importance for neutrophil function in vivo, since adherence is a prerequisite for diapedesis and chemotaxis.

AB - Changes in the cytosolic free Ca2+ concentration ([Ca2+]i) upon activation of human neutrophils by opsonized particles (serum-treated zymosan; STZ) were evaluated by three different methods: (i) measurement of total fluorescence changes in indo-1 loaded neutrophils activated in suspension; (ii) measurement of fluorescence changes in individual indo-1 loaded neutrophils in a flow cytometer and (iii) measurement of fluorescence changes in individual fura-2 loaded neutrophils adherent to serum-coated coverslips. Our study shows that the opsonized particle-induced change in [Ca2+]i in neutrophils is altered during adherence of the cells to a serum-coated surface. These observations might be of importance for neutrophil function in vivo, since adherence is a prerequisite for diapedesis and chemotaxis.

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Adherence of human neutrophils changes Ca²⁺ signaling during activation with opsonized particles

Abstract

Keywords

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