@article{6a9a41a701e948df94f251c90cdfe601,
title = "Activation-induced colocalisation of SCAMP5 with IFNα in human plasmacytoid dendritic cells",
abstract = "INTRODUCTION: Plasmacytoid dendritic cells (pDCs) are the main producers of type I interferon (IFN) in SLE. pDCs express high secretory carrier membrane protein 5 (SCAMP5). Recent work in transfected HEK cells connects SCAMP5 to the type I IFN secretory pathway. To further study the role of SCAMP5 in IFNα secretion by pDCs, we focused on the subcellular distribution of SCAMP5 in human pDCs freshly isolated from peripheral blood.METHODS: We measured SCAMP5 expression by flow cytometry in peripheral blood mononuclear cells of healthy subjects (n=8). Next, we assessed the colocalisation of SCAMP5 with IFNα in pDCs of healthy subjects (n=4) by evaluating bright detail similarity (BDS) scores using ImageStream technology.RESULTS: We confirm that SCAMP5 is highly expressed by pDCs derived from peripheral blood. In activated pDCs, we show that SCAMP5 colocalises with IFNα (mean BDS 2.0±0.1; BDS >2.0 in 44\% of pDCs).CONCLUSION: SCAMP5 colocalises with IFNα in activated human pDCs, in support of a role of this trafficking protein in the secretion of type I IFN by pDCs.",
keywords = "autoimmunity, interferon type I, lupus erythematosus, systemic",
author = "Pouw, \{Juli{\"e}tte N\} and \{Olde Nordkamp\}, \{Michel A M\} and O'Toole, \{Tom G\} and Radstake, \{Timothy R D J\} and Leijten, \{Emmerik F A\} and Marianne Boes",
note = "Funding Information: Contributors TRDJR and MB were responsible for conception. JNP, EFAL and MB designed the experiments. JNP and MAMON performed the experiments. JNP and TGO'T were responsible for the analyses. JNP and MB primarily wrote the manuscript. All authors contributed to substantial discussion of content, and review and revision of the manuscript before submission. Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors. Competing interests MB reports research grants from Actuate Therapeutics, Nutricia and Argenx, unrelated to the submitted work. All other authors state no conflict of interest and have no disclosures. Patient consent for publication Not required. Ethics approval This study involves human participants. At University Medical Center Utrecht, a cohort of healthy subjects is used from the Mini Donor Service (MDS). The MDS aims to collect blood from healthy volunteers to support scientific research and laboratory diagnosis at UMC Utrecht. Donors are volunteers who by means of a written declaration have indicated their willingness to donate small quantities of blood (100 mL maximum) four times a year without being reimbursed by financial or other means. The work has been carried out in accordance with the Code of Ethics of the World Medical Association (Declaration of Helsinki). Provenance and peer review Not commissioned; externally peer reviewed. Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise. Publisher Copyright: {\textcopyright} Author(s) (or their employer(s)) 2022.",
year = "2022",
month = mar,
day = "16",
doi = "10.1136/lupus-2022-000680",
language = "English",
volume = "9",
pages = "1--3",
journal = "Lupus science \& medicine",
issn = "2053-8790",
publisher = "BMJ Publishing Group",
number = "1",
}