Absolute first trimester cell-free DNA levels and their associations with adverse pregnancy outcomes

Florentine F Thurik; Marije Lamain-de Ruiter; Ahmad Javadi; Anneke Kwee; Heleen Woortmeijer; Godelieve C M L Page-Christiaens; Arie Franx; C Ellen van der Schoot; Maria P H Koster

doi:10.1002/pd.4940

Absolute first trimester cell-free DNA levels and their associations with adverse pregnancy outcomes

Florentine F Thurik, Marije Lamain-de Ruiter, Ahmad Javadi, Anneke Kwee, Heleen Woortmeijer, Godelieve C M L Page-Christiaens, Arie Franx, C Ellen van der Schoot, Maria P H Koster

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

OBJECTIVE: To study associations of first trimester cell-free fetal DNA levels (in this paper referred to as cell-free placental DNA (cfpDNA) levels) and preeclampsia (PE), pregnancy-induced hypertension (PIH), gestational diabetes (GDM) and spontaneous preterm birth (sPB).

METHOD: A nested case-control study was conducted in first trimester samples (gestational age 8(+0) -13(+6) weeks). A total of 226 cases and 301 controls were included. CfpDNA levels were quantified in male-bearing pregnancies using real-time DYS14-PCRs on DNA isolated from maternal serum. CfpDNA multiples of the median (MoMs) were calculated based on associations with patient characteristics (body mass index, parity, ethnicity and smoking). Associations between MoMs and adverse outcomes were studied.

RESULTS: Cell-free placental DNA levels were negatively associated with body mass index (β = -0.297, p < 0.001) and smoking (β = -0.163, p = 0.006). MoMs were lower in women who later developed PIH (n = 84, p = 0.009) or GDM (n = 56, p = 0.037). There was no association between cfpDNA MoMs and PE (n = 37, p = 0.15) or sPB (n = 49, p = 0.19). CfpDNA was positively correlated with pregnancy-associated plasma protein A (r = 0.426, p < 0.001) but not with placental growth factor (r = 0.059, p = 0.179).

Original language	English
Pages (from-to)	1104–1111
Journal	Prenatal Diagnosis
Volume	36
Issue number	12
DOIs	https://doi.org/10.1002/pd.4940
Publication status	Published - 2016

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@article{f530bee88b844fd69da8c8c01544480e,

title = "Absolute first trimester cell-free DNA levels and their associations with adverse pregnancy outcomes",

abstract = "OBJECTIVE: To study associations of first trimester cell-free fetal DNA levels (in this paper referred to as cell-free placental DNA (cfpDNA) levels) and preeclampsia (PE), pregnancy-induced hypertension (PIH), gestational diabetes (GDM) and spontaneous preterm birth (sPB).METHOD: A nested case-control study was conducted in first trimester samples (gestational age 8(+0) -13(+6) weeks). A total of 226 cases and 301 controls were included. CfpDNA levels were quantified in male-bearing pregnancies using real-time DYS14-PCRs on DNA isolated from maternal serum. CfpDNA multiples of the median (MoMs) were calculated based on associations with patient characteristics (body mass index, parity, ethnicity and smoking). Associations between MoMs and adverse outcomes were studied.RESULTS: Cell-free placental DNA levels were negatively associated with body mass index (β = -0.297, p < 0.001) and smoking (β = -0.163, p = 0.006). MoMs were lower in women who later developed PIH (n = 84, p = 0.009) or GDM (n = 56, p = 0.037). There was no association between cfpDNA MoMs and PE (n = 37, p = 0.15) or sPB (n = 49, p = 0.19). CfpDNA was positively correlated with pregnancy-associated plasma protein A (r = 0.426, p < 0.001) but not with placental growth factor (r = 0.059, p = 0.179).CONCLUSION: Adjusted first trimester cfpDNA levels are associated with PIH and GDM but probably not with PE or sPB. {\textcopyright} 2016 John Wiley & Sons, Ltd.",

author = "Thurik, {Florentine F} and {Lamain-de Ruiter}, Marije and Ahmad Javadi and Anneke Kwee and Heleen Woortmeijer and Page-Christiaens, {Godelieve C M L} and Arie Franx and {van der Schoot}, {C Ellen} and Koster, {Maria P H}",

note = "{\textcopyright} 2016 John Wiley & Sons, Ltd.",

year = "2016",

doi = "10.1002/pd.4940",

language = "English",

volume = "36",

pages = "1104–1111",

journal = "Prenatal Diagnosis",

issn = "0197-3851",

publisher = "John Wiley & Sons Inc.",

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TY - JOUR

T1 - Absolute first trimester cell-free DNA levels and their associations with adverse pregnancy outcomes

AU - Thurik, Florentine F

AU - Lamain-de Ruiter, Marije

AU - Javadi, Ahmad

AU - Kwee, Anneke

AU - Woortmeijer, Heleen

AU - Page-Christiaens, Godelieve C M L

AU - Franx, Arie

AU - van der Schoot, C Ellen

AU - Koster, Maria P H

PY - 2016

Y1 - 2016

N2 - OBJECTIVE: To study associations of first trimester cell-free fetal DNA levels (in this paper referred to as cell-free placental DNA (cfpDNA) levels) and preeclampsia (PE), pregnancy-induced hypertension (PIH), gestational diabetes (GDM) and spontaneous preterm birth (sPB).METHOD: A nested case-control study was conducted in first trimester samples (gestational age 8(+0) -13(+6) weeks). A total of 226 cases and 301 controls were included. CfpDNA levels were quantified in male-bearing pregnancies using real-time DYS14-PCRs on DNA isolated from maternal serum. CfpDNA multiples of the median (MoMs) were calculated based on associations with patient characteristics (body mass index, parity, ethnicity and smoking). Associations between MoMs and adverse outcomes were studied.RESULTS: Cell-free placental DNA levels were negatively associated with body mass index (β = -0.297, p < 0.001) and smoking (β = -0.163, p = 0.006). MoMs were lower in women who later developed PIH (n = 84, p = 0.009) or GDM (n = 56, p = 0.037). There was no association between cfpDNA MoMs and PE (n = 37, p = 0.15) or sPB (n = 49, p = 0.19). CfpDNA was positively correlated with pregnancy-associated plasma protein A (r = 0.426, p < 0.001) but not with placental growth factor (r = 0.059, p = 0.179).CONCLUSION: Adjusted first trimester cfpDNA levels are associated with PIH and GDM but probably not with PE or sPB. © 2016 John Wiley & Sons, Ltd.

AB - OBJECTIVE: To study associations of first trimester cell-free fetal DNA levels (in this paper referred to as cell-free placental DNA (cfpDNA) levels) and preeclampsia (PE), pregnancy-induced hypertension (PIH), gestational diabetes (GDM) and spontaneous preterm birth (sPB).METHOD: A nested case-control study was conducted in first trimester samples (gestational age 8(+0) -13(+6) weeks). A total of 226 cases and 301 controls were included. CfpDNA levels were quantified in male-bearing pregnancies using real-time DYS14-PCRs on DNA isolated from maternal serum. CfpDNA multiples of the median (MoMs) were calculated based on associations with patient characteristics (body mass index, parity, ethnicity and smoking). Associations between MoMs and adverse outcomes were studied.RESULTS: Cell-free placental DNA levels were negatively associated with body mass index (β = -0.297, p < 0.001) and smoking (β = -0.163, p = 0.006). MoMs were lower in women who later developed PIH (n = 84, p = 0.009) or GDM (n = 56, p = 0.037). There was no association between cfpDNA MoMs and PE (n = 37, p = 0.15) or sPB (n = 49, p = 0.19). CfpDNA was positively correlated with pregnancy-associated plasma protein A (r = 0.426, p < 0.001) but not with placental growth factor (r = 0.059, p = 0.179).CONCLUSION: Adjusted first trimester cfpDNA levels are associated with PIH and GDM but probably not with PE or sPB. © 2016 John Wiley & Sons, Ltd.

U2 - 10.1002/pd.4940

DO - 10.1002/pd.4940

M3 - Article

C2 - 27734510

SN - 0197-3851

VL - 36

SP - 1104

EP - 1111

JO - Prenatal Diagnosis

JF - Prenatal Diagnosis

IS - 12

ER -

Absolute first trimester cell-free DNA levels and their associations with adverse pregnancy outcomes

Abstract

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