Absence of chemokine (C-X-C Motif) ligand 10 diminishes perfusion recovery after local arterial occlusion in mice

P. van den Borne; R.T. Haverslag; M. Brandt; C. Cheng; H.J. Duckers; P.H. Quax; I.E. Hoefer; G. Pasterkamp; D.P.V. de Kleijn

doi:10.1161/ATVBAHA.113.303050

Absence of chemokine (C-X-C Motif) ligand 10 diminishes perfusion recovery after local arterial occlusion in mice

P. van den Borne, R.T. Haverslag, M. Brandt, C. Cheng, H.J. Duckers, P.H. Quax, I.E. Hoefer, G. Pasterkamp^*, D.P.V. de Kleijn

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

10 Citations (Scopus)

Abstract

OBJECTIVE - : In arteriogenesis, pre-existing anastomoses undergo enlargement to restore blood flow in ischemic tissues. Chemokine (C-X-C motif) ligand 10 (CXCL10) is secreted after Toll-like receptor activation. Toll-like receptors are involved in arteriogenesis; however, the role of CXCL10 is still unclear. In this study, we investigated the role for CXCL10 in a murine hindlimb ischemia model. APPROACH AND RESULTS - : Unilateral femoral artery ligation was performed in wild-type (WT) and CXCL10 knockout (KO) mice and perfusion recovery was measured using laser-Doppler perfusion analysis. Perfusion recovery was significantly lower in KO mice compared with WT at days 4 and 7 after surgery (KO versus WT: 28±5% versus 81±13% at day 4; P=0.003 and 57±12% versus 107±8% at day 7; P=0.003). Vessel measurements of α-smooth muscle actin-positive vessels revealed increasing numbers in time after surgery, which was significantly higher in WT when compared with that in KO. Furthermore, α-smooth muscle actin-positive vessels were significantly larger in WT when compared with those in KO at day 7 (wall thickness, P

Original language	English
Pages (from-to)	594-602
Number of pages	9
Journal	Arteriosclerosis, Thrombosis and Vascular Biology
Volume	34
Issue number	3
DOIs	https://doi.org/10.1161/ATVBAHA.113.303050
Publication status	Published - Mar 2014

Keywords

Cell movement
Chemokine CXCL10
Inflammation
interferon-γ-inducible protein of 10 kDa
Myocytes, smooth muscle

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10.1161/ATVBAHA.113.303050

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van den Borne, P., Haverslag, R. T., Brandt, M., Cheng, C., Duckers, H. J., Quax, P. H., Hoefer, I. E., Pasterkamp, G., & de Kleijn, D. P. V. (2014). Absence of chemokine (C-X-C Motif) ligand 10 diminishes perfusion recovery after local arterial occlusion in mice. Arteriosclerosis, Thrombosis and Vascular Biology, 34(3), 594-602. https://doi.org/10.1161/ATVBAHA.113.303050

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title = "Absence of chemokine (C-X-C Motif) ligand 10 diminishes perfusion recovery after local arterial occlusion in mice",

abstract = "OBJECTIVE - : In arteriogenesis, pre-existing anastomoses undergo enlargement to restore blood flow in ischemic tissues. Chemokine (C-X-C motif) ligand 10 (CXCL10) is secreted after Toll-like receptor activation. Toll-like receptors are involved in arteriogenesis; however, the role of CXCL10 is still unclear. In this study, we investigated the role for CXCL10 in a murine hindlimb ischemia model. APPROACH AND RESULTS - : Unilateral femoral artery ligation was performed in wild-type (WT) and CXCL10 knockout (KO) mice and perfusion recovery was measured using laser-Doppler perfusion analysis. Perfusion recovery was significantly lower in KO mice compared with WT at days 4 and 7 after surgery (KO versus WT: 28±5% versus 81±13% at day 4; P=0.003 and 57±12% versus 107±8% at day 7; P=0.003). Vessel measurements of α-smooth muscle actin-positive vessels revealed increasing numbers in time after surgery, which was significantly higher in WT when compared with that in KO. Furthermore, α-smooth muscle actin-positive vessels were significantly larger in WT when compared with those in KO at day 7 (wall thickness, P",

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author = "{van den Borne}, P. and R.T. Haverslag and M. Brandt and C. Cheng and H.J. Duckers and P.H. Quax and I.E. Hoefer and G. Pasterkamp and {de Kleijn}, D.P.V.",

year = "2014",

month = mar,

doi = "10.1161/ATVBAHA.113.303050",

language = "English",

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pages = "594--602",

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AU - van den Borne, P.

AU - Haverslag, R.T.

AU - Brandt, M.

AU - Cheng, C.

AU - Duckers, H.J.

AU - Quax, P.H.

AU - Hoefer, I.E.

AU - Pasterkamp, G.

AU - de Kleijn, D.P.V.

PY - 2014/3

Y1 - 2014/3

N2 - OBJECTIVE - : In arteriogenesis, pre-existing anastomoses undergo enlargement to restore blood flow in ischemic tissues. Chemokine (C-X-C motif) ligand 10 (CXCL10) is secreted after Toll-like receptor activation. Toll-like receptors are involved in arteriogenesis; however, the role of CXCL10 is still unclear. In this study, we investigated the role for CXCL10 in a murine hindlimb ischemia model. APPROACH AND RESULTS - : Unilateral femoral artery ligation was performed in wild-type (WT) and CXCL10 knockout (KO) mice and perfusion recovery was measured using laser-Doppler perfusion analysis. Perfusion recovery was significantly lower in KO mice compared with WT at days 4 and 7 after surgery (KO versus WT: 28±5% versus 81±13% at day 4; P=0.003 and 57±12% versus 107±8% at day 7; P=0.003). Vessel measurements of α-smooth muscle actin-positive vessels revealed increasing numbers in time after surgery, which was significantly higher in WT when compared with that in KO. Furthermore, α-smooth muscle actin-positive vessels were significantly larger in WT when compared with those in KO at day 7 (wall thickness, P

AB - OBJECTIVE - : In arteriogenesis, pre-existing anastomoses undergo enlargement to restore blood flow in ischemic tissues. Chemokine (C-X-C motif) ligand 10 (CXCL10) is secreted after Toll-like receptor activation. Toll-like receptors are involved in arteriogenesis; however, the role of CXCL10 is still unclear. In this study, we investigated the role for CXCL10 in a murine hindlimb ischemia model. APPROACH AND RESULTS - : Unilateral femoral artery ligation was performed in wild-type (WT) and CXCL10 knockout (KO) mice and perfusion recovery was measured using laser-Doppler perfusion analysis. Perfusion recovery was significantly lower in KO mice compared with WT at days 4 and 7 after surgery (KO versus WT: 28±5% versus 81±13% at day 4; P=0.003 and 57±12% versus 107±8% at day 7; P=0.003). Vessel measurements of α-smooth muscle actin-positive vessels revealed increasing numbers in time after surgery, which was significantly higher in WT when compared with that in KO. Furthermore, α-smooth muscle actin-positive vessels were significantly larger in WT when compared with those in KO at day 7 (wall thickness, P

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KW - Inflammation

KW - interferon-γ-inducible protein of 10 kDa

KW - Myocytes, smooth muscle

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ER -

Absence of chemokine (C-X-C Motif) ligand 10 diminishes perfusion recovery after local arterial occlusion in mice

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