TY - JOUR
T1 - Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts
AU - Van Den Bossche, Lindsay
AU - Schoonenberg, Vivien A.C.
AU - Burgener, Iwan A.
AU - Penning, Louis C.
AU - Schrall, Ingrid M.
AU - Kruitwagen, Hedwig S.
AU - Van Wolferen, Monique E.
AU - Grinwis, Guy C.M.
AU - Kummeling, Anne
AU - Rothuizen, Jan
AU - Van Velzen, Jeroen F.
AU - Stathonikos, Nikolas
AU - Molenaar, Martijn R.
AU - Helms, Bernd J.
AU - Brouwers, Jos F.H.M.
AU - Spee, Bart
AU - Van Steenbeek, Frank G.
N1 - Publisher Copyright:
© 2017 Van den Bossche et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2017/10/19
Y1 - 2017/10/19
N2 - Non-alcoholic fatty liver disease (NAFLD) is a poorly understood multifactorial pandemic disorder. One of the hallmarks of NAFLD, hepatic steatosis, is a common feature in canine congenital portosystemic shunts. The aim of this study was to gain detailed insight into the pathogenesis of steatosis in this large animal model. Hepatic lipid accumulation, gene-expression analysis and HPLC-MS of neutral lipids and phospholipids in extrahepatic (EHPSS) and intrahepatic portosystemic shunts (IHPSS) was compared to healthy control dogs. Liver organoids of diseased dogs and healthy control dogs were incubated with palmitic- and oleic-acid, and lipid accumulation was quantified using LD540. In histological slides of shunt livers, a 12-fold increase of lipid content was detected compared to the control dogs (EHPSS P<0.01; IHPSS P = 0.042). Involvement of lipid-related genes to steatosis in portosystemic shunting was corroborated using gene-expression profiling. Lipid analysis demonstrated different triglyceride composition and a shift towards short chain and omega-3 fatty acids in shunt versus healthy dogs, with no difference in lipid species composition between shunt types. All organoids showed a similar increase in triacylglycerols after free fatty acids enrichment. This study demonstrates that steatosis is probably secondary to canine portosystemic shunts. Unravelling the pathogenesis of this hepatic steatosis might contribute to a better understanding of steatosis in NAFLD.
AB - Non-alcoholic fatty liver disease (NAFLD) is a poorly understood multifactorial pandemic disorder. One of the hallmarks of NAFLD, hepatic steatosis, is a common feature in canine congenital portosystemic shunts. The aim of this study was to gain detailed insight into the pathogenesis of steatosis in this large animal model. Hepatic lipid accumulation, gene-expression analysis and HPLC-MS of neutral lipids and phospholipids in extrahepatic (EHPSS) and intrahepatic portosystemic shunts (IHPSS) was compared to healthy control dogs. Liver organoids of diseased dogs and healthy control dogs were incubated with palmitic- and oleic-acid, and lipid accumulation was quantified using LD540. In histological slides of shunt livers, a 12-fold increase of lipid content was detected compared to the control dogs (EHPSS P<0.01; IHPSS P = 0.042). Involvement of lipid-related genes to steatosis in portosystemic shunting was corroborated using gene-expression profiling. Lipid analysis demonstrated different triglyceride composition and a shift towards short chain and omega-3 fatty acids in shunt versus healthy dogs, with no difference in lipid species composition between shunt types. All organoids showed a similar increase in triacylglycerols after free fatty acids enrichment. This study demonstrates that steatosis is probably secondary to canine portosystemic shunts. Unravelling the pathogenesis of this hepatic steatosis might contribute to a better understanding of steatosis in NAFLD.
UR - http://www.scopus.com/inward/record.url?scp=85031735006&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0186491
DO - 10.1371/journal.pone.0186491
M3 - Article
AN - SCOPUS:85031735006
SN - 1932-6203
VL - 12
SP - 1
EP - 17
JO - PLoS ONE
JF - PLoS ONE
IS - 10
M1 - e0186491
ER -