A thermo-responsive and photo-polymerizable chondroitin sulfate-based hydrogel for 3D printing applications

A. Abbadessa; M. M. Blokzijl; V. H. M. Mouser; P. Marica; J. Malda; W. E. Hennink; T. Vermonden

doi:10.1016/j.carbpol.2016.04.080

A thermo-responsive and photo-polymerizable chondroitin sulfate-based hydrogel for 3D printing applications

A. Abbadessa
, M. M. Blokzijl
, V. H. M. Mouser
, P. Marica
, J. Malda
, W. E. Hennink
, T. Vermonden

Regenerative Medicine and Stem Cells

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The aim ofthis study was to design a hydrogel system based on methacrylated chondroitin sulfate (CSMA)
and a thermo-sensitive poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate)-polyethylene glycol
triblock copolymer (M15P10) as a suitable material for additive manufacturing of scaffolds. CSMA was
synthesized by reaction of chondroitin sulfate with glycidyl methacrylate (GMA) in dimethylsulfoxide
at 50 ◦C and its degree of methacrylation was tunable up to 48.5%, by changing reaction time and GMA
feed. Unlike polymer solutions composed of CSMA alone (20% w/w), mixtures based on 2% w/w of CSMA
and 18% of M15P10 showed strain-softening, thermo-sensitive and shear-thinning properties more pronounced
than those found for polymer solutions based on M15P10 alone. Additionally, they displayed a
yield stress of 19.2 ± 7.0 Pa. The 3D printing of this hydrogel resulted in the generation of constructs with
tailorable porosity and good handling properties. Finally, embedded chondrogenic cells remained viable
and proliferating over a culture period of 6 days. The hydrogel described herein represents a promising
biomaterial for cartilage 3D printing applications.

Original language	English
Pages (from-to)	163-174
Journal	Carbohydrate polymers
Volume	149
DOIs	https://doi.org/10.1016/j.carbpol.2016.04.080
Publication status	Published - 20 Sept 2016

Keywords

Methacrylated chondroitin sulfate
Thermo-sensitive hydrogel
Photo-polymerization
Shear thinning
Cartilage 3D printing

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10.1016/j.carbpol.2016.04.080

57X-mainFinal published version, 2.23 MBLicence: Taverne

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title = "A thermo-responsive and photo-polymerizable chondroitin sulfate-based hydrogel for 3D printing applications",

abstract = "The aim ofthis study was to design a hydrogel system based on methacrylated chondroitin sulfate (CSMA) and a thermo-sensitive poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate)-polyethylene glycol triblock copolymer (M15P10) as a suitable material for additive manufacturing of scaffolds. CSMA was synthesized by reaction of chondroitin sulfate with glycidyl methacrylate (GMA) in dimethylsulfoxide at 50 ◦C and its degree of methacrylation was tunable up to 48.5\%, by changing reaction time and GMA feed. Unlike polymer solutions composed of CSMA alone (20\% w/w), mixtures based on 2\% w/w of CSMA and 18\% of M15P10 showed strain-softening, thermo-sensitive and shear-thinning properties more pronounced than those found for polymer solutions based on M15P10 alone. Additionally, they displayed a yield stress of 19.2 ± 7.0 Pa. The 3D printing of this hydrogel resulted in the generation of constructs with tailorable porosity and good handling properties. Finally, embedded chondrogenic cells remained viable and proliferating over a culture period of 6 days. The hydrogel described herein represents a promising biomaterial for cartilage 3D printing applications.",

keywords = "Methacrylated chondroitin sulfate, Thermo-sensitive hydrogel, Photo-polymerization, Shear thinning, Cartilage 3D printing",

author = "A. Abbadessa and Blokzijl, \{M. M.\} and Mouser, \{V. H. M.\} and P. Marica and J. Malda and Hennink, \{W. E.\} and T. Vermonden",

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AU - Abbadessa, A.

AU - Blokzijl, M. M.

AU - Mouser, V. H. M.

AU - Marica, P.

AU - Malda, J.

AU - Hennink, W. E.

AU - Vermonden, T.

PY - 2016/9/20

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N2 - The aim ofthis study was to design a hydrogel system based on methacrylated chondroitin sulfate (CSMA) and a thermo-sensitive poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate)-polyethylene glycol triblock copolymer (M15P10) as a suitable material for additive manufacturing of scaffolds. CSMA was synthesized by reaction of chondroitin sulfate with glycidyl methacrylate (GMA) in dimethylsulfoxide at 50 ◦C and its degree of methacrylation was tunable up to 48.5%, by changing reaction time and GMA feed. Unlike polymer solutions composed of CSMA alone (20% w/w), mixtures based on 2% w/w of CSMA and 18% of M15P10 showed strain-softening, thermo-sensitive and shear-thinning properties more pronounced than those found for polymer solutions based on M15P10 alone. Additionally, they displayed a yield stress of 19.2 ± 7.0 Pa. The 3D printing of this hydrogel resulted in the generation of constructs with tailorable porosity and good handling properties. Finally, embedded chondrogenic cells remained viable and proliferating over a culture period of 6 days. The hydrogel described herein represents a promising biomaterial for cartilage 3D printing applications.

AB - The aim ofthis study was to design a hydrogel system based on methacrylated chondroitin sulfate (CSMA) and a thermo-sensitive poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate)-polyethylene glycol triblock copolymer (M15P10) as a suitable material for additive manufacturing of scaffolds. CSMA was synthesized by reaction of chondroitin sulfate with glycidyl methacrylate (GMA) in dimethylsulfoxide at 50 ◦C and its degree of methacrylation was tunable up to 48.5%, by changing reaction time and GMA feed. Unlike polymer solutions composed of CSMA alone (20% w/w), mixtures based on 2% w/w of CSMA and 18% of M15P10 showed strain-softening, thermo-sensitive and shear-thinning properties more pronounced than those found for polymer solutions based on M15P10 alone. Additionally, they displayed a yield stress of 19.2 ± 7.0 Pa. The 3D printing of this hydrogel resulted in the generation of constructs with tailorable porosity and good handling properties. Finally, embedded chondrogenic cells remained viable and proliferating over a culture period of 6 days. The hydrogel described herein represents a promising biomaterial for cartilage 3D printing applications.

KW - Methacrylated chondroitin sulfate

KW - Thermo-sensitive hydrogel

KW - Photo-polymerization

KW - Shear thinning

KW - Cartilage 3D printing

U2 - 10.1016/j.carbpol.2016.04.080

DO - 10.1016/j.carbpol.2016.04.080

M3 - Article

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SP - 163

EP - 174

JO - Carbohydrate polymers

JF - Carbohydrate polymers

ER -

A thermo-responsive and photo-polymerizable chondroitin sulfate-based hydrogel for 3D printing applications

Abstract

Keywords

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