Abstract
The aim ofthis study was to design a hydrogel system based on methacrylated chondroitin sulfate (CSMA)
and a thermo-sensitive poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate)-polyethylene glycol
triblock copolymer (M15P10) as a suitable material for additive manufacturing of scaffolds. CSMA was
synthesized by reaction of chondroitin sulfate with glycidyl methacrylate (GMA) in dimethylsulfoxide
at 50 ◦C and its degree of methacrylation was tunable up to 48.5%, by changing reaction time and GMA
feed. Unlike polymer solutions composed of CSMA alone (20% w/w), mixtures based on 2% w/w of CSMA
and 18% of M15P10 showed strain-softening, thermo-sensitive and shear-thinning properties more pronounced
than those found for polymer solutions based on M15P10 alone. Additionally, they displayed a
yield stress of 19.2 ± 7.0 Pa. The 3D printing of this hydrogel resulted in the generation of constructs with
tailorable porosity and good handling properties. Finally, embedded chondrogenic cells remained viable
and proliferating over a culture period of 6 days. The hydrogel described herein represents a promising
biomaterial for cartilage 3D printing applications.
and a thermo-sensitive poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate)-polyethylene glycol
triblock copolymer (M15P10) as a suitable material for additive manufacturing of scaffolds. CSMA was
synthesized by reaction of chondroitin sulfate with glycidyl methacrylate (GMA) in dimethylsulfoxide
at 50 ◦C and its degree of methacrylation was tunable up to 48.5%, by changing reaction time and GMA
feed. Unlike polymer solutions composed of CSMA alone (20% w/w), mixtures based on 2% w/w of CSMA
and 18% of M15P10 showed strain-softening, thermo-sensitive and shear-thinning properties more pronounced
than those found for polymer solutions based on M15P10 alone. Additionally, they displayed a
yield stress of 19.2 ± 7.0 Pa. The 3D printing of this hydrogel resulted in the generation of constructs with
tailorable porosity and good handling properties. Finally, embedded chondrogenic cells remained viable
and proliferating over a culture period of 6 days. The hydrogel described herein represents a promising
biomaterial for cartilage 3D printing applications.
| Original language | English |
|---|---|
| Pages (from-to) | 163-174 |
| Journal | Carbohydrate polymers |
| Volume | 149 |
| DOIs | |
| Publication status | Published - 20 Sept 2016 |
Keywords
- Methacrylated chondroitin sulfate
- Thermo-sensitive hydrogel
- Photo-polymerization
- Shear thinning
- Cartilage 3D printing