A Robust Post-Insertion Method for the Preparation of Targeted Lipid Nanoparticles

Jaime Fernández Bimbo; Raymond Schiffelers

doi:10.1007/978-1-0716-4394-5_18

A Robust Post-Insertion Method for the Preparation of Targeted Lipid Nanoparticles

Jaime Fernández Bimbo^*
, Raymond Schiffelers

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Lipid nanoparticles (LNPs) have garnered significant attention as nonviral vectors for gene delivery due to their low immunogenicity and cytotoxicity. In addition, their surfaces can readily be functionalized with targeting moieties. Here we describe a simple and robust method for the preparation of targeted siRNA, mRNA, or DNA LNPs. After conjugating a targeting ligand to a PEG lipid, this binder-lipid conjugate is post-inserted into preformed LNPs. This process allows the LNPs to be easily decorated with one or various combinations of ligands.

Original language	English
Pages (from-to)	241-250
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2901
DOIs	https://doi.org/10.1007/978-1-0716-4394-5_18
Publication status	Published - 3 Apr 2025

Keywords

DNA/chemistry
Gene Transfer Techniques
Humans
Lipids/chemistry
Liposomes
Nanoparticles/chemistry
Polyethylene Glycols/chemistry
RNA, Messenger/genetics
RNA, Small Interfering/genetics

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10.1007/978-1-0716-4394-5_18

A Robust Post-Insertion Method for the Preparation of Targeted Lipid NanoparticlesFinal published version, 610 KBLicence: Taverne

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abstract = "Lipid nanoparticles (LNPs) have garnered significant attention as nonviral vectors for gene delivery due to their low immunogenicity and cytotoxicity. In addition, their surfaces can readily be functionalized with targeting moieties. Here we describe a simple and robust method for the preparation of targeted siRNA, mRNA, or DNA LNPs. After conjugating a targeting ligand to a PEG lipid, this binder-lipid conjugate is post-inserted into preformed LNPs. This process allows the LNPs to be easily decorated with one or various combinations of ligands.",

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AU - Bimbo, Jaime Fernández

AU - Schiffelers, Raymond

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PY - 2025/4/3

Y1 - 2025/4/3

N2 - Lipid nanoparticles (LNPs) have garnered significant attention as nonviral vectors for gene delivery due to their low immunogenicity and cytotoxicity. In addition, their surfaces can readily be functionalized with targeting moieties. Here we describe a simple and robust method for the preparation of targeted siRNA, mRNA, or DNA LNPs. After conjugating a targeting ligand to a PEG lipid, this binder-lipid conjugate is post-inserted into preformed LNPs. This process allows the LNPs to be easily decorated with one or various combinations of ligands.

AB - Lipid nanoparticles (LNPs) have garnered significant attention as nonviral vectors for gene delivery due to their low immunogenicity and cytotoxicity. In addition, their surfaces can readily be functionalized with targeting moieties. Here we describe a simple and robust method for the preparation of targeted siRNA, mRNA, or DNA LNPs. After conjugating a targeting ligand to a PEG lipid, this binder-lipid conjugate is post-inserted into preformed LNPs. This process allows the LNPs to be easily decorated with one or various combinations of ligands.

KW - DNA/chemistry

KW - Gene Transfer Techniques

KW - Humans

KW - Lipids/chemistry

KW - Liposomes

KW - Nanoparticles/chemistry

KW - Polyethylene Glycols/chemistry

KW - RNA, Messenger/genetics

KW - RNA, Small Interfering/genetics

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U2 - 10.1007/978-1-0716-4394-5_18

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SN - 1064-3745

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

A Robust Post-Insertion Method for the Preparation of Targeted Lipid Nanoparticles

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