TY - JOUR
T1 - A Robust Post-Insertion Method for the Preparation of Targeted Lipid Nanoparticles
AU - Bimbo, Jaime Fernández
AU - Schiffelers, Raymond
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025/4/3
Y1 - 2025/4/3
N2 - Lipid nanoparticles (LNPs) have garnered significant attention as nonviral vectors for gene delivery due to their low immunogenicity and cytotoxicity. In addition, their surfaces can readily be functionalized with targeting moieties. Here we describe a simple and robust method for the preparation of targeted siRNA, mRNA, or DNA LNPs. After conjugating a targeting ligand to a PEG lipid, this binder-lipid conjugate is post-inserted into preformed LNPs. This process allows the LNPs to be easily decorated with one or various combinations of ligands.
AB - Lipid nanoparticles (LNPs) have garnered significant attention as nonviral vectors for gene delivery due to their low immunogenicity and cytotoxicity. In addition, their surfaces can readily be functionalized with targeting moieties. Here we describe a simple and robust method for the preparation of targeted siRNA, mRNA, or DNA LNPs. After conjugating a targeting ligand to a PEG lipid, this binder-lipid conjugate is post-inserted into preformed LNPs. This process allows the LNPs to be easily decorated with one or various combinations of ligands.
KW - DNA/chemistry
KW - Gene Transfer Techniques
KW - Humans
KW - Lipids/chemistry
KW - Liposomes
KW - Nanoparticles/chemistry
KW - Polyethylene Glycols/chemistry
KW - RNA, Messenger/genetics
KW - RNA, Small Interfering/genetics
U2 - 10.1007/978-1-0716-4394-5_18
DO - 10.1007/978-1-0716-4394-5_18
M3 - Article
C2 - 40175880
SN - 1064-3745
VL - 2901
SP - 241
EP - 250
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -